Abcam, ab115514, Anti-Pleckstrin antibody

Size: 50µg
Goat Polyclonal Pleckstrin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PLEK aa 300 to C-terminus.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PLEK aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P08567

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: 99% Tris buffered saline, 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pleckstrin also known as PLEK is a phosphoprotein with a mass of approximately 40 kDa. It is predominantly expressed in platelets and leukocytes. Pleckstrin is the major protein kinase C substrate in platelets and acts as an important mediator in signal transduction processes. Its structure includes pleckstrin homology domains which play key roles in interacting with other cellular proteins and in lipid binding.
Biological function summary
Pleckstrin participates in cellular processes like cytoskeletal rearrangement and cell motility. It functions as part of protein signaling complexes that regulate actin cytoskeleton dynamics. Through its pleckstrin homology domains pleckstrin binds to phosphatidylinositol lipids which is critical for recruitment to membrane sites required for cellular activation and response.
Pathways
Pleckstrin is intricately involved in the PI3K/AKT signaling pathway and the platelet activation pathway. Within these pathways pleckstrin interacts with proteins like G-protein coupled receptors and PI3K facilitating cellular responses to external stimuli. These processes ensure platelets function correctly during hemostasis and inflammatory responses.
Pleckstrin's role is critical in diseases like thrombocytopenia and psoriasis. Aberrations in pleckstrin expression or function can lead to improper platelet function contributing to abnormal bleeding or clotting scenarios in thrombocytopenia. It is also connected with proteins like PIP3 and AKT which are involved in pathways that can become dysregulated in chronic inflammatory disorders such as psoriasis.