Abcam, ab116027, Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

Size: 500µL
10-180 kDa prestained protein ladder (ab116027) is a ready- to-use, three-color protein standard with 10 pre-stained proteins ranging from molecular weights 10 to 180 kDa. - Suitable for western blot (WB) and SDS-PAGE - Suitable for Tris Glycine (4-12%) SDS buffer, Bis-Tris (4-12%) MOPS buffer and Bis-Tris (4-12%) MES buffer - Part of our Lab essential range western blot workflow
Key facts
Applications:WB, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Form:LiquidSee storage information,
Storage buffer:pH: 7.5Constituents: 15% Glycerol (glycerin, glycerine), 3% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 2% Sodium lauryl sulfate, 1% Tris phosphate, 0.02% Urea

Product details:
Prestained Protein Ladder ab116027 is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).
This product was previously called Prism Ultra Protein Ladder (10 - 180 kDa).
This prestained protein ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use.
Do not heat, dilute, add reducing agent before loading.
Key product features:
Broad range:
10-180 kDa
Ready-to-use:
Supplied in a loading buffer for direct loading on gels.
Easy to identify:
Includes green ~25 kDa and red ~75kDa reference bands.
Sharp bands
Recommended loading:
~2 - 3 μl for western blots
Review other protein ladders in the
unstained and prestained protein ladder guide

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C