Abcam, ab11822, Anti-Coilin antibody [Pdelta]

Size: 50µL
Mouse Monoclonal Coilin antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Recombinant full length protein - Mouse samples. Cited in 44 publications. Immunogen corresponding to Recombinant Fragment Protein within Human COIL.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:Pdelta,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human COIL. The exact immunogen used to generate this antibody is proprietary information.P38432,
Epitope:Monoclonal Anti-coilin reacts with an epitope located between amino acids 363 and 481 of human coilin.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Coilin also known as Coilin protein or its gene name COIL is a nuclear phosphoprotein with a mass of approximately 62 kDa. It plays a mechanical role in the formation and maintenance of Cajal bodies which are subnuclear structures involved in various RNA processes. Coilin is mainly expressed in the nucleus of eukaryotic cells including a high expression in HeLa cells where its localization to Cajal bodies can be easily studied with tools like Alexa Fluor 488. Occasionally references may be made to terms like 'HeLa coil' which pertain to specific structural features noted in these contexts.
Biological function summary
This protein facilitates the organization and biogenesis of nuclear bodies associated with RNA processing and small nuclear ribonucleoproteins (snRNPs) assembly. Coilin operates as part of a larger complex involving several other proteins that interact within Cajal bodies. These nuclear bodies serve as sites where components necessary for RNA processing are concentrated. Coilin aids in the trafficking and modification of snRNP elements which are essential for pre-mRNA splicing.
Pathways
Coilin affects RNA metabolism and processing notably within the spliceosome assembly pathway and in snRNP biogenesis. The pathways include interactions with proteins such as SMN (Survival of Motor Neuron) protein which links to spinal muscular atrophy-related processes. Additionally Coilin contributes to the broader framework of nucleic acid processing systems impacting the outcome of RNA transcription and modification through its regulatory roles.
Coilin shows connections to neurological conditions and cancer. For instance alterations or dysregulation in Coilin expression can correlate with spinal muscular atrophy where it associates with the SMN protein if there are disruptions in Cajal body integrity. Furthermore links exist with certain cancers where the mismanagement of RNA processes influenced by Coilin and its interaction partners like PDEdelta can lead to aberrant cell behaviors and tumor progression. Coilin’s involvement in such diseases makes it an appealing target for therapeutic exploration reflected in ongoing cancer research and therapeutic developments.