Abcam, ab11871, Anti-C3 / C3b antibody [755]

Size: 50µg
Anti-C3 / C3b antibody [755] (ab11871) is a mouse monoclonal antibody detecting C3 / C3b in Western Blot, IHC-P, ICC/IF . Suitable for Cynomolgus monkey, Human . - Over 20 publications - Trusted since 2004
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:755,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Human, Cynomolgus monkey,
Applications:IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Human C3.P01024,
Specificity:This antibody reacts with C3 and C3b. See References multiple isoforms detected by WB.

Product details:
What is this antibody validated in?
Anti-C3 / C3b antibody [755] (ab11871) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Cynomolgus monkey, Human samples.
Trusted by the scientific community
Anti-C3 / C3b [755] (ab11871) was first used in a scientific publication in 2004 and has been cited over 20 times in peer-reviewed journals.
Reviewed by scientists
Anti-C3 / C3b [755] (ab11871) has over 5 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C3 also known as complement C3 or anti C3b is a central protein in the complement system with an approximate molecular mass of 187 kDa. This protein exists in blood plasma as an inactive precursor and is produced mainly in the liver. C3 gets activated through cleavage into two fragments C3a and C3b. The functional fragment C3b covalently attaches to pathogen surfaces through a process called opsonization allowing it to 'tag' pathogens for destruction by immune cells.
Biological function summary
Complement C3b plays an integral role in innate immunity. C3b is a part of the complement cascade forming complexes such as C3bBb which acts as a convertase in the amplification of the complement response. Through opsonization C3b promotes the binding and uptake of pathogens by phagocytes therefore enhancing immune defense.
Pathways
C3b integrates into the alternative and classical complement pathways. In these pathways C3b binds with factors like B and D contributing to the formation of the C5 convertase and progressing to the membrane attack complex. These interactions involve proteins such as C5 and Factor B essential for the effective destruction of pathogens and the clearance of immune complexes.
Complement C3b shows associations with diseases involving improper immune regulation such as atypical hemolytic uremic syndrome (aHUS) and age-related macular degeneration (AMD). Mutations affecting C3b's interactions with regulatory proteins such as Factor H can lead to uncontrolled complement activation which contributes to the pathology of these diseases. Understanding these interactions provides insights into therapeutic targets for managing complement-related diseases.