Abcam, ab11878, Anti-C5a / C5a des Arg antibody [2952]

Size: 50µg
Mouse Monoclonal C5 antibody. Suitable for ELISA, WB, IHC-P, FuncS (Neut/Block), IHC-Fr and reacts with Human samples. Cited in 7 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:2952,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:FuncS (Neut/Block), WB, ELISA, IHC-P, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:This antibody reacts with a neo-epitope (des-Arg) on C5a that is formed upon cleavage of C5 into C5a and C5b. It will detect C5a and C5a des-Arg (Not C5).Please notice that under given conditions it is known that C5 can expose epitopes normally only found in the cleaved activation products.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-0.2 µm filtered, Storage buffer-Constituents: PBS, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The C5a protein also known as anaphylatoxin C5a is a small potent molecule weighing approximately 11 kDa. It originates from the larger complement component C5 during complement system activation. The C5a des Arg is a slightly modified version produced when enzymes cleave the C-terminal arginine from C5a. Both forms express on cell surfaces and recognize specific receptors on target cells particularly in immune and inflammatory responses.
Biological function summary
This protein controls complex interactions within the immune system specifically as part of the cleavage product of C5 a component of the complement system. C5a binds to receptors such as C5aR and C5L2 which appear in high numbers on various immune cells like macrophages neutrophils and endothelial cells. This binding triggers multiple intracellular pathways mediating inflammatory processes and immune cell activation.
Pathways
The complement system involves the activation sequences where C5a plays a pivotal role. It participates in the activation of the classic and the alternative complement pathways contributing to the opsonization and lysis of pathogens. C5a works closely with proteins such as C3a and C9 orchestrating the body's defense mechanisms against infections while facilitating communication between the innate and adaptive immune systems.
C5a plays a notable role in inflammatory diseases like rheumatoid arthritis and sepsis. Its overactivation relates to severe inflammatory responses and tissue damage. The link between C5a and other inflammatory mediators such as tumor necrosis factor-alpha (TNF-alpha) emphasizes its contribution to the pathogenesis of these conditions. Understanding these pathways offers insight into potential therapeutic targets for modulating excessive inflammation and immune responses.