Abcam, ab118796, Anti-CENPI antibody

Size: 100µg
Rabbit Polyclonal CENPI antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CENPI aa 50-100.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human CENPI aa 50-100. The exact immunogen used to generate this antibody is proprietary information.Q92674

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CENPI also known as centromere protein I is part of the CENP family and plays a mechanical role in centromere assembly and function. It has a molecular mass of approximately 97 kDa. CENPI is expressed in various tissues but it shows higher expression levels in proliferating cells especially during cell division. It localizes to the centromere region of chromosomes and is essential for the proper functioning of the kinetochore the protein structure on chromosomes where spindle fibers attach during cell division.
Biological function summary
The role of CENPI involves its participation in the CENP-A nucleosome-associated complex. This complex is essential for the recruitment of other kinetochore proteins ensuring accurate chromosome segregation during mitosis. The proper functioning of CENPI contributes to maintaining genomic stability as errors in chromosome segregation can lead to aneuploidy which is an abnormal number of chromosomes in cells. Therefore CENPI acts as an important mediator in the accurate distribution of chromosomes during cell division.
Pathways
CENPI plays a significant role in the cell cycle regulation and mitotic checkpoint pathways. It aids in the activation and stability of proteins like CENP-H and CENP-K which cooperate during the early stages of centromere and kinetochore formation. This activity ensures that the assembling of the mitotic spindle is well-regulated and that cells do not prematurely progress through mitosis. Properly functioning CENPI and its pathway partners are critical to preventing cell division errors that could lead to various cellular dysfunctions.
Malfunction or aberrant expression of CENPI has been linked to certain cancers such as colorectal and breast cancer. Disrupted CENPI function can lead to improper chromosome segregation contributing to genetic instability and tumor progression. Additionally studies have pointed to connections between CENPI dysregulation and CENP-E another centromere protein. Together abnormalities in these proteins can exacerbate the risk of oncogenesis and promote aggressive tumor behavior highlighting the importance of the CENP family in controlling cell cycle fidelity and preventing disease development.