Abcam, ab119259, Anti-NAPE-PLD antibody [OTI3D5]

Size: 100µL
Mouse Monoclonal NAPE-PLD antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NAPEPLD.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:OTI3D5,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human NAPEPLD.Q6IQ20

Product details:
Clone OTI3D5 (formerly 3D5).

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-Purified from cell culture supernatant by affinity chromatography., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NAPE-PLD also known as N-acyl phosphatidylethanolamine phospholipase D is an enzyme with a molecular mass of approximately 46 kDa. It functions in hydrolyzing N-acyl phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs) which include bioactive lipids like anandamide. NAPE-PLD is expressed widely in mammalian tissues such as the brain and liver indicating its broad physiological significance.
Biological function summary
NAPE-PLD plays an important role in generating bioactive lipids that act as signaling molecules. This enzyme is not known to be part of a larger protein complex but functions independently to influence lipid-based signaling pathways. By modulating the levels of NAEs it affects various physiological processes including inflammation and neuroprotection.
Pathways
The ability of NAPE-PLD to produce NAEs integrates it into the endocannabinoid signaling pathway a critical route for maintaining homeostasis in the body. It works alongside other enzymes like fatty acid amide hydrolase (FAAH) that break down NAEs. The activity of NAPE-PLD influences cannabinoid receptor activity impacting several pharmacological responses and regulatory mechanisms in the nervous system.
Involvement of NAPE-PLD is significant in conditions such as neurodegenerative diseases and metabolic disorders. Alterations in its activity have been linked to Alzheimer's disease where it affects endocannabinoid levels. The enzyme also connects to obesity-related disorders through its impact on energy balance and lipid metabolism alongside proteins like diacylglycerol lipase which also participate in lipid signaling networks.