Abcam, ab119930, Anti-DLK-1 antibody [3A10]

Size: 100µL
Mouse Monoclonal DLK-1 antibody. Suitable for Flow Cyt, ELISA, WB, ICC/IF and reacts with Mouse, Human, Synthetic peptide, Recombinant full length protein samples. Cited in 16 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DLK1 aa 150-350.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:3A10,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:ELISA, Flow Cyt, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human DLK1 aa 150-350. The exact immunogen used to generate this antibody is proprietary information.P80370

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from tissue culture supernatant., Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Delta-like 1 homolog (DLK-1) also known as Pref-1 (preadipocyte factor 1) is a protein involved in a wide range of cellular processes. The mass of DLK-1 is approximately 65 kDa. This protein is expressed in various tissues including the liver brain and endocrine glands. DLK-1 has an important role in the regulation of cell differentiation and growth acting as a signal molecule that influences tissue development and regeneration.
Biological function summary
DLK-1 serves a function in the inhibition of adipocyte differentiation and the maintenance of preadipocyte phenotype. It does not form part of a complex but interacts with membrane-bound receptors to mediate signaling pathways that impact tissue development. Researchers study its role in embryonic development and its downregulation is a significant factor during the differentiation of mesenchymal cells. Due to these interactions DLK-1 has a pivotal involvement in processes governing tissue regeneration and growth control.
Pathways
Better understanding of DLK-1 involves examining its interaction in the Notch signaling pathway. This important pathway related to cell fate decisions features pathways with other proteins like Notch-1 and Notch-3. The relationship helps modulate the fate and proliferation of cells. DLK-1 also has associations with pathways involving phosphoinositide 3-kinase (PI3K) which influences cell growth and survival through related proteins such as AKT.
DLK-1 has shown associations with cancer and metabolic disorders. Overexpression of DLK-1 is linked to obesity due to its impact on preadipocyte differentiation. Additionally higher levels of DLK-1 can correlate with certain cancer progression where it acts alongside proteins like Notch-1 in promoting tumor cell growth. Understanding DLK-1's role in these contexts highlights the importance of DLK-1 as both a diagnostic marker and potential therapeutic target.