Abcam, ab119942, Anti-IRAK4 antibody [2H9]

Size: 100µL
Anti-IRAK4 antibody [2H9] (ab119942) is a mouse monoclonal antibody detecting IRAK4 in Western Blot, Flow Cytometry, IHC-P, ICC/IF, ELISA . Suitable for African green monkey, Human, Mouse, . - KO validated for confirmed specificity
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:2H9,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Human, African green monkey,
Applications:ICC/IF, Flow Cyt, WB, IHC-P, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human IRAK4 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.Q9NWZ3

Product details:
What is this antibody validated in?
Anti-IRAK4 antibody [2H9] (ab119942) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in African green monkey, Human, Mouse, samples.
What is the molecular weight of IRAK4?
Anti-IRAK4 [2H9] (ab119942) specifically detects a band for IRAK4 (UniProt: Q9NWZ3) at a molecular weight of 52kDa.
Specificity confirmed
The specificity of Anti-IRAK4 antibody [2H9] (ab119942) has been confirmed by Western blot testing in IRAK4 Knockout THP-1 cell line,
ab281630

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from tissue culture supernatant., Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
IRAK4 also known as Interleukin-1 receptor-associated kinase 4 is an important protein with an approximate molecular mass of 51 kDa. This protein is a significant part of the signaling cascade initiated by the Interleukin-1 receptor (IL-1R) and Toll-like receptors (TLRs). Expressed in a variety of tissues including the spleen thymus and lung IRAK4 is involved in innate immune responses. The presence of IRAK4 protein facilitates the downstream signaling pathways that activate the transcription factors responsible for inflammatory cytokine production.
Biological function summary
IRAK4 plays an important role in mediating signaling in the innate immune system by forming complexes with other proteins such as IRAK-1. These complexes propagate signals that result in the activation of transcription factors like NF-kB and AP-1 which regulate the expression of inflammatory genes. Through these mechanisms IRAK4 influences the body's ability to respond to infections and stresses. Its critical involvement in these signaling pathways highlights its importance in maintaining immune homeostasis.
Pathways
IRAK4 is a pivotal player in the Toll-like receptor and IL-1 receptor signaling pathways which are central to the host defense against pathogens. It functions upstream activating the MyD88-dependent pathway resulting in the recruitment and phosphorylation of other kinases such as IRAK-1 and TRAF6. These interactions trigger the NF-kB signaling cascade thereby enhancing pro-inflammatory cytokine production that is essential for initiating immune responses.
Mutations or dysregulation of IRAK4 are linked to increased susceptibility to recurrent bacterial infections and sepsis. Patients with IRAK4 deficiency have impaired NF-kB activation leading to a weakened inflammatory response. Additionally overexpression of IRAK4 has been observed in certain autoimmune diseases such as rheumatoid arthritis where the persistent activation of TLR signaling contributes to chronic inflammation. The role of IRAK4 in these disorders ties closely with its interaction with proteins like TRAF6 influencing disease development and progression.