Abcam, ab122186, Anti-EPG5 antibody

Size: 100µL
Rabbit Polyclonal EPG5 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EPG5 aa 800-950.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human EPG5 aa 800-950. The exact immunogen used to generate this antibody is proprietary information.Q9HCE0

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EPG5 also known as ectopic P-granules autophagy protein 5 homolog is an important protein involved in autophagy. The protein has a mass of approximately 2572 amino acids. Expression of EPG5 occurs in various tissues with notable levels in the central nervous system. The protein participates in the regulation of autophagosome maturation which is essential for cellular homeostasis and the degradation of damaged organelles and proteins.
Biological function summary
EPG5 plays a significant role in the formation and maturation of autophagosomes acting as a tethering factor between autophagosomes and lysosomes. EPG5 does not function alone; it is part of a larger complex contributing specifically to the late stages of autophagy. This interaction helps ensure the proper fusion of autophagosomes with lysosomes enabling the degradation and recycling of cellular components.
Pathways
EPG5 is a critical component in the autophagy pathway. It interacts directly with RAB7 a small GTPase that is important for vesicle trafficking during autophagosome maturation. This interaction facilitates the fusion of autophagosomes with lysosomes. EPG5 also has ties with the endocytosis pathway highlighting its role in maintaining cellular homeostasis through these interconnected systems.
EPG5 mutations associate with Vici syndrome a rare genetic disorder characterized by multisystem symptoms including agenesis of the corpus callosum and immune deficiency. EPG5 is linked to neurodegenerative diseases as its malfunction can lead to impaired neuronal autophagy and accumulation of protein aggregates. In Vici syndrome the dysfunction of EPG5 disrupts normal autophagic processes and lysosomal activity possibly due to its interactions with other proteins such as RAB7 compounding the disease pathology.