Abcam, ab124574, Recombinant Mycobacterium tuberculosis ESAT6 protein

Size: 50µg
Recombinant Mycobacterium tuberculosis ESAT6 protein is a Mycobacterium tuberculosis (strain CDC1551) Full Length protein, in the 1 to 95 aa range, expressed in Baculovirus, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.The predicted molecular weight of ab124574 protein is 11 kDa.
Key facts
Purity:>90% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:Baculovirus,
Tags:His tag C-Terminus,
Applications:SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P9WNK6,
Animal free:No,
Carrier free:No,
Species:Mycobacterium tuberculosis CDC1551,
Storage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl

Product details:
Ensure the validity of your result using our recombinant M. tuberculosis ESAT6 protein ab124574 as positive control in SDS-PAGE.
Check out our protein gel staining guide for SDS-PAGE

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ESAT-6 protein also known as Early Secretory Antigenic Target 6 kDa is a small highly conserved protein found in Mycobacterium tuberculosis with a molecular mass of approximately 6 kDa. It is secreted by the bacterium and plays a significant role in its pathogenicity. ESAT-6 is expressed strongly during infection particularly within the host macrophages. Its ability to modulate host immune responses makes it an important molecule in tuberculosis research and diagnostics.
Biological function summary
ESAT-6 interacts with the host cell's membranes promoting virulence by disrupting immune functions. It does not act alone but forms a complex with the CFP-10 protein which is essential for its stability and secretion. This complex enables ESAT-6 to interfere with immune recognition helping Mycobacterium tuberculosis evade host defenses. These interactions make ESAT-6 a target of protein assays and a focal point in the study of mycobacterium-host interactions.
Pathways
ESAT-6 interferes with immune response pathways particularly the path leading to cell-mediated immunity. It directly affects the NF-kB pathway a critical regulator of immune response by destabilizing macrophage lysosomal membranes. The activity of ESAT-6 connects with another protein PhoP involved in regulating virulent gene expression in Mycobacterium tuberculosis. This interaction disrupts normal cellular signaling aiding in bacterial survival within the host.
ESAT-6 plays a pivotal role in the pathogenesis of tuberculosis a major infectious disease worldwide. The protein's role in attenuating immune responses makes it vital for the persistence of Mycobacterium tuberculosis within the host. Additionally ESAT-6 has connections to other disorders like granuloma formation which results from chronic infections. In tuberculosis the interplay between ESAT-6 and the Rv3879c antigen another virulence factor impacts granuloma development making it a target for therapeutic interventions aimed at combating tuberculosis.