Abcam, ab124695, Anti-ADAM10 antibody [EPR5622]

Size: 20µL / 100µL / 1mL
Anti-ADAM10 antibody [EPR5622] (ab124695) is a rabbit monoclonal antibody detecting ADAM10 in Western Blot, IP . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 40 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5622,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-ADAM10 antibody [EPR5622] (ab124695) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Human, Mouse, Rat samples.
What is the molecular weight of ADAM10?
Anti-ADAM10 [EPR5622] (ab124695) specifically detects a band for ADAM10 (UniProt: O14672) at a molecular weight of 84kDa.
Trusted by the scientific community
Anti-ADAM10 [EPR5622] (ab124695) was first used in a scientific publication in 2012 and has been cited over 40 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Specificity confirmed
The specificity of Anti-ADAM10 antibody [EPR5622] (ab124695) has been confirmed by Western blot testing in ADAM10 Knockout HAP1 cells.
Other related products
We have a range of other formats of antibody clone [EPR5622] also available for your convenience: ab124695, Carrier free -
ab242389
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Shipping conditions update: ambient shipping
This product will be delivered at ambient temperature instead of chilled – this is by design. Extensive stability testing confirmed that our products are suitable for shipment under ambient conditions and maintain expected quality.
Why the change?
It’s part of our commitment to more sustainable packaging solutions, with ambient deliveries using eco-friendly materials such as recyclable cardboard instead of polystyrene.
What you need to know
Ambient shipments come clearly marked on the delivery note.
No ice will be included in ambient shipments, but mixed orders (ambient and cold-chain items) will still arrive with ice packs to protect temperature-sensitive products.
Warranty coverage remains fully valid, aligned with our validated shipping method.
Please store the product as per the datasheet instructions upon receipt.
Find out more -
https://www.abcam.com/en-us/support/shipping-storage-support/ambient-shipping

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Conditional Ambient, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ADAM10 protein also known as CD156c is a member of the ADAM (a disintegrin and metalloprotease) family. It functions as a metalloproteinase enzyme involved in the ectodomain shedding of various cell surface proteins. ADAM10 has an approximate molecular weight of 85 kDa. This protein has broad expression in various tissues including the brain heart and liver and performs key enzymatic activities that affect cellular communication.
Biological function summary
ADAM10 contributes to development and regulation of synapses in the central nervous system being part of the membrane protein complex. It processes amyloid precursor protein (APP) and other substrates excising their extracellular domains and influencing cell signaling pathways. ADAM10 activity regulates Notch signaling a mechanism essential for cell differentiation and tissue homeostasis.
Pathways
ADAM10 activity intersects with several important biological processes including the Notch and Epidermal Growth Factor Receptor (EGFR) pathways. Notch signaling pathway involves interactions with ligands like Jagged and Delta impacting cell fate decisions. In the EGFR pathway ADAM10 regulates the availability of ligands such as EGF affecting the receptor's signaling cascades related to cell proliferation and survival.
ADAM10 dysregulation associates with Alzheimer's disease and cancer. ADAM10's role in cleaving APP is linked to Alzheimer's where altered activity may affect amyloid-beta production and plaque formation. In cancer ADAM10 modulates cell adhesion and migration influencing tumor progression and metastasis. ADAM10 interactions with proteins in these contexts such as Β-Catenin in cancer illustrate its impact on pathophysiological processes.