Abcam, ab124711, Anti-ATG10 antibody [EPR4804]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal ATG10 antibody. Suitable for WB and reacts with Human samples. Cited in 16 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4804,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
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Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATG10 also known as Autophagy-Related Protein 10 is an important player in the autophagy pathway. This protein weighs approximately 21 kDa and is expressed in a variety of tissues with high levels observed in the heart and skeletal muscle. Mechanically ATG10 acts as an E2-like enzyme important for the conjugation of ATG12 to ATG5 forming the ATG12–ATG5 conjugate essential for autophagosome formation.
Biological function summary
The autophagy process involves ATG10 in the covalent attachment of ATG12 to ATG5 a step critical for elongation and completion of autophagosomes. This protein functions as part of the ATG12–ATG5–ATG16L1 complex which plays a structural role in the phagophore membrane. Without ATG10's activity efficient autophagosome assembly cannot occur highlighting its importance in maintaining cellular homeostasis through regulated turnover of cellular components.
Pathways
ATG10 significantly impacts the macroautophagy pathway and links to metabolic control pathways. It interacts closely with proteins such as ATG5 and ATG7 which are necessary for initiating and executing the autophagy process. This involvement ensures the proper degradation of damaged organelles and recycling of nutrients during cellular stress.
Researchers have connected ATG10 activity to conditions like neurodegenerative diseases and cancers. Dysregulation of ATG10 can impair autophagic function which may contribute to Alzheimer's disease progression by preventing the clearance of protein aggregates. Additionally alteration in ATG10 expression has associations with certain cancers where it impacts cancer cell survival and proliferation. Understanding ATG10's interactions with other proteins like BECN1 could further elucidate its role in these diseases.