Abcam, ab125187, Anti-SAP30 antibody

Size: 100µg
Rabbit Polyclonal SAP30 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human Histone deacetylase complex subunit SAP30 aa 150 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human Histone deacetylase complex subunit SAP30 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.O75446

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SAP30 also known as Sin3-associated protein 30 is a component of the Sin3A corepressor complex which modulates gene transcription. This protein has a molecular mass of approximately 30 kDa. SAP30 localizes mainly in the nucleus where it interacts with DNA and other proteins. It is expressed in a wide variety of cell types highlighting its broad regulatory roles in cells.
Biological function summary
SAP30 participates in chromatin remodeling and regulation of gene expression by recruiting histone deacetylases (HDACs) as part of the Sin3A complex. This complex is integral to the repression of transcription during cell cycle regulation and response to DNA damage. By forming a bridge between the Sin3A complex and chromatin SAP30 ensures the recruitment of these enzymes facilitating the removal of acetyl groups from histones which leads to a tighter chromatin structure and repressed gene transcription.
Pathways
SAP30 plays important roles in transcriptional repression pathways and cell cycle regulation. It associates closely with the Sin3A-HDAC complex in these pathways along with proteins such as HDAC1 and HDAC2. The action of SAP30 in these pathways influences cell cycle checkpoints and transcriptional silencing making it an important regulator in maintaining genomic stability and controlling cell proliferation.
SAP30 has associations with cancer and neurodegenerative disorders. Alterations in its regulatory functions can disrupt normal gene expression contributing to tumorigenesis. SAP30 connects with proteins such as p53 and Rb which are often implicated in cancer development. In neurodegenerative disorders the dysregulated expression of genes affected by SAP30 can influence neuronal survival hinting at its potential role in diseases like Alzheimer's where HDAC inhibitors are explored for therapeutic interventions.