Abcam, ab126057, Anti-IRSp53 antibody

Size: 50µL
Rabbit Polyclonal IRSp53 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Brain-specific angiogenesis inhibitor 1-associated protein 2 aa 1-200.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human Brain-specific angiogenesis inhibitor 1-associated protein 2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.Q9UQB8

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
IRSp53 also known as BAIAP2 (Brain-Specific Angiogenesis Inhibitor 1-Associated Protein 2) is a protein with a molecular mass of approximately 58 kDa. It acts as an important regulator of actin cytoskeleton dynamics facilitating the formation of membrane protrusions like filopodia and lamellipodia. IRSp53 is expressed ubiquitously in various tissues with higher levels observed in the brain. It contains several domains including the IMD (I-BAR) domain which is involved in membrane curvature and actin binding.
Biological function summary
IRSp53 participates in the modulation of cell shape and motility. It is a component of the protein complexes that couple membrane dynamics to cytoskeleton remodeling. Through its interaction with proteins like CDC42 and Rac1 IRSp53 guides the rearrangement of actin filaments affecting cell movement and shape. These abilities make it significant for cellular processes requiring changes in cell structure such as neurodevelopment and wound healing.
Pathways
IRSp53 is central to the regulation of cell motility pathways and signaling processes. It plays an important role in the WAVE (WASP family verprolin-homologous protein) regulatory complex which influences actin polymerization. The protein interacts with Rac1 and N-WASP to mediate pathways leading to the formation of actin-rich structures. This modulation of actin dynamics links IRSp53 to the broader MAPK/ERK signaling pathway impacting cellular proliferation and differentiation.
IRSp53 has been linked to neurological and oncological conditions. Alterations in its expression or function have connections with autism spectrum disorders and glioblastoma. In these disorders IRSp53's interaction with proteins like BAIAP2L1 can influence signal transduction and cytoskeletal organization which may contribute to disease pathology. Understanding IRSp53's roles could offer insights into its potential as a therapeutic target.