Abcam, ab126243, Anti-UHRF1 antibody

Size: 50µL
Rabbit Polyclonal UHRF1 antibody. Suitable for IP, ChIP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UHRF1 aa 500-750.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, IP, ICC/IF, ChIP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human UHRF1 aa 500-750. The exact immunogen used to generate this antibody is proprietary information.Q96T88

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
UHRF1 also known as ICBP90 or Np95 is a nuclear protein playing key roles in epigenetic regulation. It weighs approximately 90 kDa. UHRF1 is expressed in various tissues most highly in proliferating cells. Functionally UHRF1 recognizes hemimethylated DNA and recruits DNA methyltransferase 1 (DNMT1) to maintain DNA methylation after replication. This ability to interact with both DNA and chromatin makes UHRF1 central to chromatin modification processes.
Biological function summary
UHRF1 engages in preserving the DNA methylation landscape ensuring proper gene expression patterns. It is part of a multi-protein complex and processes both histone modification and DNA methylation. Its presence is critical for gene silencing through the recruitment of DNMT1 and histone deacetylases. UHRF1 therefore influences regulatory regions of genes important for cell cycle progression and development.
Pathways
UHRF1 integrates into methylation-dependent gene silencing and cell cycle regulation. It actively participates in DNA damage repair pathways by modulating the p53 pathway aiding cell survival post-DNA damage. UHRF1 closely interacts with epigenetic regulators such as DNMT1 and histone deacetylases bridging methylation and chromatin remodeling to ensure proper cell cycle and transcriptional control.
UHRF1's dysregulation links to cancers including breast and lung cancer due to its role in maintaining aberrant methylation. Abnormal UHRF1 expression leads to improper silencing of tumor suppressor genes. UHRF1's interaction with DNMT1 and modulation of pathways involving p53 are important for cancer cell proliferation making it a potential target for cancer therapies.