Abcam, ab126824, Anti-Troponin I fast skeletal muscle antibody [2F12G2]

Size: 100µL
Mouse Monoclonal Troponin I fast skeletal muscle antibody. Suitable for Flow Cyt, ELISA, WB, ICC/IF and reacts with Mouse, Human, Recombinant fragment samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TNNI2.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:2F12G2,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:Flow Cyt, ICC/IF, ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human TNNI2.P48788

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from tissue culture supernatant., Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Troponin I fast skeletal muscle also known as TNNI2 is a protein involved in muscle contraction. It weighs around 21.2 kDa and is mainly expressed in fast-twitch skeletal muscle. TNNI2 acts by inhibiting actomyosin ATPase activity meaning it helps prevent muscle contraction until it receives a signal to contract. It binds to actin and plays a regulatory role in the muscle contraction process.
Biological function summary
Troponin I fast skeletal muscle interacts within the troponin complex which includes Troponin T and Troponin C. Located on the thin filament of the muscle fiber this complex modulates the contraction of striated muscle by controlling calcium binding. When calcium binds to Troponin C it induces a conformational change that displaces TNNI2 from actin allowing for muscle contraction to occur.
Pathways
Troponin I fast skeletal muscle has essential functions in the calcium modulation pathways specific to muscle contraction. It plays an important role in the regulation of muscle contraction by interacting with calcium ions and other proteins such as tropomyosin. As part of the excitation-contraction coupling pathway TNNI2 helps ensure the proper contraction of muscles in response to neural stimulation. Additionally its role in these pathways involves specific interactions with proteins like Troponin C and Troponin T which are necessary for functional muscle contraction.
Abnormalities in Troponin I fast skeletal muscle can lead to muscle-related conditions such as nemaline myopathy and distal arthrogryposis. These disorders affect muscle structure and function leading to muscle weakness or deformities. Mutations in TNNI2 may result in improper interaction with other sarcomeric proteins such as tropomyosin disrupting normal muscle contraction and leading to the mentioned diseases. Understanding the role of TNNI2 in these disorders can aid in the development of diagnostic and therapeutic strategies.