Abcam, ab126863, Anti-SIM1 antibody

Size: 200µL
Goat Polyclonal SIM1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SIM1 aa 500-600.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human SIM1 aa 500-600. The exact immunogen used to generate this antibody is proprietary information.P81133

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: 99% Tris buffered saline, 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SIM1 also known as Single-minded homolog 1 is a transcription factor with a mass of approximately 83 kDa. This protein plays a mechanical role in regulating gene expression by binding to specific DNA sequences through its basic helix-loop-helix (bHLH) and PAS domains. SIM1 is expressed in several tissues including the hypothalamus where it contributes to the development of specific neuronal populations.
Biological function summary
The role of SIM1 extends to neurodevelopment where it interacts with other transcription factors to form complexes essential for brain functioning. Notably SIM1 is critical for the proper formation and differentiation of neurons in the paraventricular nucleus of the hypothalamus which are important for physiological regulation and neuroendocrine functions. Through these interactions SIM1 influences various processes related to behavior and homeostasis.
Pathways
The role of SIM1 becomes evident in developmental and signaling pathways. It is involved in the hypothalamic-pituitary axis where it interacts with melanocortin 4 receptor (MC4R) signaling. These pathways are significant for regulating food intake and energy expenditure. SIM1 also has associations with the Fibroblast Growth Factor (FGF) pathway influencing the terminal differentiation of specific neuron types.
The dysfunction of SIM1 can lead to conditions such as obesity and developmental brain disorders. Mutations or altered expression of SIM1 link it to obesity emphasizing its connection with energy balance and weight control through its interaction with MC4R. Furthermore abnormalities in SIM1 expression may relate to idiopathic hypogonadotropic hypogonadism associated with disrupted interactions with other hypothalamic factors.