Abcam, ab1269, HRP Anti-6X His tag® antibody

Size: 100µg
Anti-6X His tag® antibody - HRP conjugated (ab1269) is a goat polyclonal antibody detecting 6X His tag® in Western Blot, ELISA . - Over 10 publications - Trusted since 2002
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Conjugation:HRP,
Carrier free:No,
Applications:ICC, ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
What is this antibody validated in?
HRP Anti-6X His tag® antibody (ab1269) is a goat polyclonal antibody and is validated for use in Western Blot (WB), ELISA.
Tagged protein detection
6X His tag® antibodies are used to visualize proteins labelled with this tag in a variety of applications.
Trusted by the scientific community
Anti-6X His tag® HRP (ab1269) was first used in a scientific publication in 2002 and has been cited over 10 times in peer-reviewed journals.
HIS-TAG® is a trademark of EMD Biosciences, Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG., Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.
Biological function summary
Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.
Pathways
The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.
The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.