Abcam, ab127617, Recombinant human PI3K(p110alpha/p55gamma) protein (Active)

Size: 5µg / 10µg
Recombinant human PI3K(p110alpha/p55gamma) protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, WB, FuncS.
Key facts
Purity:>70% Densitometry,
Expression system:Baculovirus infected Sf9 cells,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, FuncS, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:420 nmol/min/mg.,
Accession:P42336,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7Preservative: 1.02% ImidazoleConstituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PI3K p110alpha/p55gamma often referred to as PI3Kα is a member of the phosphoinositide 3-kinases (PI3Ks) family. It plays a significant role in intracellular signal transduction. PI3Kα is a heterodimer composed of a p110 catalytic subunit and a p55 regulatory subunit. The molecular mass of the p110alpha subunit is approximately 124 kDa while the p55gamma regulatory subunit has a mass of around 55 kDa. PI3Kα expresses mainly in tissues with high cellular proliferation rates such as liver heart and muscle indicating its involvement in key biological processes.
Biological function summary
PI3Kα regulates cellular functions by participating in lipid phosphorylation at the 3-position of the inositol ring producing phosphatidylinositol (345)-trisphosphate (PIP3). This is an important event in recruiting signaling proteins with PH domains to the membrane leading to the activation of kinases like AKT. PI3Kα usually forms part of a complex with other proteins in order to facilitate its functions. This complex arrangement aids in mediating cell growth proliferation survival and metabolic responses making it a central player in cellular physiology.
Pathways
PI3Kα integrates into the PI3K/AKT/mTOR pathway a critical signaling cascade that controls myriad cellular processes. Within this pathway PI3Kα activates AKT which in turn interacts with downstream effectors like mTOR to regulate cellular metabolism and growth. PI3Kα also connects with proteins like PTEN and PDK1 to maintain a balance between cell survival and apoptosis. Besides this pathway influences glucose homeostasis and can affect various cellular states through intricate signaling networks.
PI3Kα links strongly with cancer and insulin resistance conditions like Type 2 diabetes. Mutations and overexpression of PI3Kα have associations with several cancer types including breast and colorectal cancers by driving unchecked cellular proliferation and survival. In insulin resistance dysfunctional PI3Kα impair signaling through the insulin pathway reducing the responsiveness of cells to insulin and affecting glucose uptake. The interaction of PI3Kα with proteins such as PTEN and AKT plays an important role in these pathological conditions underlining its importance as a target in therapeutic interventions.