Abcam, ab127887, Recombinant HIV1 Gag p15 protein

Size: 100µg
Recombinant HIV1 Gag p15 protein is a Human immunodeficiency virus type 1 (NEW YORK-5 ISOLATE) Full Length protein, in the 365 to 488 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, ELISA, WB, FuncS.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:Tag free,
Applications:FuncS, ELISA, WB, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P12497,
Animal free:No,
Carrier free:No,
Species:Human immunodeficiency virus type 1 (NEW YORK-5 ISOLATE),
Storage buffer:pH: 7.5Constituents: 50% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.29% Sodium chloride, 0.078% 2-Mercaptoethanol

Product details:
ab127887 can be used as a substrate for HIV1 protease in the presence of HIV1 genomic RNA; in studies of structure and function of AIDS virus as precursor of nucleocapsid p7 protein that binds to HIV1 genome RNA; as p15 antigen in detection of anti-HIV1 p15 antibody in Western blotting or ELISA; and as a standard for the quantitative analysis of HIV1 p15 antigen.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The HIV1 Gag p15 protein also known as Gag polyprotein is an essential target in the life cycle of HIV-1. It has a molecular weight of about 15 kDa. This protein is initially produced as a part of the larger Gag polyprotein precursor in the host cell cytoplasm. After its synthesis the precursor undergoes cleavage by the viral protease enzyme to produce Gag p15 among other components. The protein predominantly localizes to the inner surface of the plasma membrane facilitating the assembly of viral particles.
Biological function summary
The structural role of Gag p15 is significant in the viral assembly and budding processes. As part of the larger Gag polyprotein complex it contributes to forming the matrix proteins necessary for virion assembly. The protein undergoes myristoylation which aids its interaction with the host cell membrane a step vital for virion particle budding and release. Its presence is key to establishing the conformation that enables the enclosed viral genome packaging during the assembly process.
Pathways
The HIV1 Gag p15 protein plays a role in the virus replication cycle and is tightly linked to the viral budding pathway. It interacts with host cell components such as the ESCRT machinery which is important for the separation of the nascent virion from the host cell membrane. Related proteins in this process include Gag p24 and Gag p7 which also derive from the Gag precursor and work together to secure viral assembly and release.
The HIV1 Gag p15 protein directly relates to HIV/AIDS progression. Its function is important for the assembly of infectious virus particles making it a target for antiretroviral therapies designed to halt viral replication. Moreover the proteins show interaction with other viral elements such as the reverse transcriptase and integrase during the viral life cycle. Understanding these interactions offers insight into potential therapeutic approaches to manage and treat HIV-related conditions.