Abcam, ab128173, Anti-GCC185 antibody

Size: 100µg
Rabbit Polyclonal GCC185 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GCC2 aa 1-50.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human GCC2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.Q8IWJ2

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab128173 is affinity purified using an epitope specific to GCC185 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GCC185 also known as Golgi coiled-coil protein 185 plays a mechanical role in the tethering of Golgi membranes. It has a mass of approximately 185 kDa and localizes to the trans-Golgi network (TGN). GCC185 is responsible for maintaining Golgi structure and integrity by interacting with various membrane vesicles and microtubules. It is broadly expressed in different cell types emphasizing its general importance in maintaining the secretory pathway across various tissues.
Biological function summary
GCC185 facilitates vesicle tethering at the Golgi apparatus. It is a component of the larger Golgin family which is a group of coiled-coil proteins. These proteins assist in modulating Golgi organization and function by interactively recruiting and tethering vesicles from the trans-Golgi to other cellular destinations. GCC185 specifically binds to the Rab GTPases contributing to membrane trafficking and ensuring the effective transport of proteins and lipids within the cell.
Pathways
The function of GCC185 integrates within the intracellular trafficking and sorting pathways. It notably participates in the trafficking of lysosomal hydrolases through the mannose-6-phosphate (M6P) receptor pathway. GCC185 interacts with other Golgin family members and proteins like Rab9 facilitating retrograde transport from endosomes to the Golgi an essential process for protein sorting and recycling.
Improper functioning of GCC185 is linked to disorders involving lysosomal storage and Golgi dysfunction. One significant condition associated with GCC185 is neurodegenerative disease where defects in protein sorting and trafficking can impair neuronal function. Moreover disruptions in the GCC185-Rab9 connection are implicated in the pathology of disorders like Alzheimer's disease as they contribute to the mistrafficking of amyloid precursor proteins further illustrating the protein's critical role in maintaining cellular homeostasis.