Abcam, ab128435, Recombinant Human TPRKB protein

Size: 50µg
Recombinant Human TPRKB protein is a Human Full Length protein, in the 1 to 175 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q9Y3C4,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TPRKB also known as TP53RK-binding protein is a protein with a mass of approximately 39 kDa. It is expressed widely in various human tissues with notable levels seen in the testis. Mechanically TPRKB operates as part of the KEOPS/EKC complex which is essential for certain cellular processes. This complex participates in tRNA modifications and plays a role in maintaining certain chromosome structures.
Biological function summary
TPRKB contributes significantly to cell growth and survival. The KEOPS/EKC complex which includes TPRKB is involved in a range of cellular activities such as the addition of threonylcarbamoyl-AMP (t6A) to tRNAs. This modification ensures proper translation of the genetic code important for protein synthesis. Through these actions TPRKB assists in regulating cell cycle and cellular responses to stress. Proper functioning of tRNA modifications is essential for accurate protein translation impacting cellular homeostasis and division.
Pathways
TPRKB is integral to both the KEOPS/EKC pathway and the mTOR signaling pathway. The proper function of these pathways is important for nutrient sensing and regulation of cell growth. TPRKB interacts with other proteins such as TP53RK within the KEOPS/EKC complex. In the mTOR signaling pathway its role contributes to nutrient sensing and energy balance. These interactions hint at TPRKB's potential involvement in cellular metabolic regulation.
TPRKB has associations with certain cancers and growth-related abnormalities. It relates to cancer through its role in cell cycle regulation and genomic stability impacting tumorigenesis processes. The interaction of TPRKB with the protein TP53RK in the KEOPS/EKC complex may affect cell cycle control and tumor suppression. Additionally changes or mutations in TPRKB can link to growth disorders due to its critical functions in tRNA modification and cell growth pathways.