Abcam, ab129002, Anti-Vinculin antibody [EPR8185] - Loading Control

Size: 20µL / 100µL / 250µL / 1mL
Anti-Vinculin antibody [EPR8185] (ab129002) is a rabbit monoclonal antibody detecting Vinculin in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 350 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR8185,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, IP, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-Vinculin antibody [EPR8185] (ab129002) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IP and WB.
Anti-Vinculin antibody [EPR8185] (ab129002) was first used in a scientific publication in 2013 and has been cited over 356 times in peer reviewed journals. It's performance in Western Blot in mouse and human samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-Vinculin antibody [EPR8185] (ab129002) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-Vinculin antibody [EPR8185] (ab129002) has been confirmed by Western Blot testing in Vinculin knockout A431 cells.
Anti-Vinculin antibody [EPR8185] (ab129002) has 21 independent reviews from customers.
Vinculin antibodies are often used as loading controls in Western Blot. Anti-Vinculin antibody [EPR8185] has been verified in Western Blot samples and detects a band at 124kDa Molecular weight.
Anti-Vinculin antibody [EPR8185] (ab129002) specifically detects Vinculin (UniProt ID: P18206; Molecular weight: 124kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL, 250 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone EPR8185 -
ab217171
Antibody clone EPR8185 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, Alexa Fluor
647, Alexa Fluor
ab196454
ab196579
ab206908
Vinculin is a focal adhesion protein that plays a critical role in cell adhesion, migration and mechanotransduction. In oncology, it influences tumor progression and metastasis by modulating the interactions between cancer cells and the extracellular matrix.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C