Abcam, ab129752, Anti-NDUFA4 antibody

Size: 100µg
Rabbit Polyclonal NDUFA4 antibody. Suitable for ELISA, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human Cytochrome c oxidase subunit NDUFA4.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:ICC/IF, WB, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human Cytochrome c oxidase subunit NDUFA4. The exact immunogen used to generate this antibody is proprietary information.O00483

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NDUFA4 also known as NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4 is a small protein with a molecular mass of around 9 kDa. It plays a role as part of the mitochondrial respiratory chain where it contributes to the electron transport process. NDUFA4 is expressed in various tissues but has higher levels of expression in tissues with elevated metabolic demand like the heart liver and muscle. Its location in the inner mitochondrial membrane allows it to participate directly in respiration activity by aiding electron transfer from NADH to ubiquinone.
Biological function summary
NDUFA4 functions as part of the larger protein complex known as Complex I or NADH:ubiquinone oxidoreductase. Complex I is a critical component of the mitochondrial electron transport chain. It supports ATP synthesis by creating a proton gradient across the inner mitochondrial membrane. NDUFA4 participates in this energy conversion process and cooperates with additional protein subunits in Complex I to maintain cellular energy metabolism. This makes it essential for maintaining efficient aerobic respiration.
Pathways
NDUFA4 has a significant role within the oxidative phosphorylation pathway a central metabolic pathway in cellular energy production. Within this pathway it associates with other proteins such as NDUFB8 and NDUFS1 in Complex I which work together for effective electron transport. NDUFA4 also intersects with the broader pathways of metabolic regulation lending support to the processes that produce ATP as a cellular energy currency.
Mutations or dysregulation of NDUFA4 have been connected to mitochondrial diseases such as mitochondrial myopathy. These conditions arise due to impaired energy production in cells. Additionally alterations in NDUFA4 levels or activity have implications in neurodegenerative disorders like Parkinson's disease. In such diseases proteins like NDUFS4 another Complex I subunit also become affected highlighting potential protein-protein interactions critical for maintaining mitochondrial health.