Abcam, ab129817, Kinetic Apoptosis Kit (Microscopy)

Size: 200µL
Kinetic Apoptosis Kit ab129817 uses pSIVA TM -IANBD to stain membrane phosphatidylserine in apoptotic cells.
Key facts
Detection method:Fluorescent,
Sample types:Suspension cells, Adherent cells,
Assay type:Quantitative,
Assay Platform:Fluorescence microscope

Product details:
Kinetic Apoptosis Kit (Microscopy) ab129817 is based on pSIVA
technology.
(Polarity Sensitive Indicator of Viability & Apoptosis)
is an Annexin XII-based polarity sensitive probe for the spatiotemporal analysis of apoptosis and other forms of cell death. pSIVA
binding is reversible which enables researchers to detect irreversible as well as transient phosphatidylserine (PS) exposure.
PS exposure is a hallmark phenomenon occurring early during apoptosis and persisting throughout the cell death process and it often considered to be irreversible. However, transient PS exposure is increasingly being recognized as a phenomenon in its own right and described to occur during both normal physiological processes and reversible or rescuable apoptotic/cell death events.
The pSIVA
assay is very straightforward: add pSIVA-IANBD or pSIVA-IANBD + Propidium Iodide (PI) directly to cells or tissues, incubate and analyze.
is conjugated to IANBD, a polarity sensitive dye that fluoresces only when pSIVA is bound to the cell membrane. pSIVA-IANBD fluorescence is measured using conventional FITC filter sets. pSIVA-IANBD applications include flow cytometry (
ab129816
) and live cell fluorescence microscopy imaging.
pSIVA-IANBD is polar sensitive. pSIVA-IANBD fluoresces in non-polar but not polar environments. When pSIVA-IANBD is bound to PS it is in the non-polar environment of the membrane lipid bilayer and fluoresces. However, when pSIVA-IANBD is not bound it is in the polar environment of the media or buffer and does not fluoresce.
The manual contains detailed information about the pSIVA technology and protocols; please read the manual prior to beginning your experiments.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C