Abcam, ab131218, Anti-CPSF30/NAR antibody [EPR7433]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal CPSF30/NAR antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR7433,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CPSF30 also known as Cleavage and Polyadenylation Specificity Factor subunit 30 is a protein involved in the cleavage and polyadenylation of pre-mRNA. It has a mass of about 30 kDa and performs its functions inside the cell nucleus. CPSF30 is expressed widely across various tissues enabling it to play multiple roles in RNA processing. Another notable alias for this protein is NAR (Nuclear Apoptosis Regulatory protein) which highlights its involvement in apoptotic processes.
Biological function summary
CPSF30 helps in mRNA processing by forming part of the CPSF complex. This complex is important for cutting and adding a poly-A tail to the 3' end of pre-mRNAs a step essential for mRNA stability export and translation. CPSF30 interacts directly with other CPSF subunits such as CPSF73 and CPSF100 enabling the PCAS endonuclease activity required for cleaving pre-mRNA. This activity facilitates proper gene expression regulation in response to different cellular conditions.
Pathways
CPSF30 is an integral part of the post-transcriptional regulation of gene expression. The protein participates in both the mRNA polyadenylation pathway and mRNA surveillance pathway ensuring accurate mRNA maturation and degradation of faulty mRNA. Through these pathways CPSF30 interacts with related proteins such as FIP1 and CSTF77 which assist in the precise control of gene expression by assuring proper RNA transcript modification and functionality.
CPSF30 involvement links to specific health conditions related to RNA processing defects and apoptosis. Disorders including certain types of cancer and autoimmune diseases have been associated with abnormalities in CPSF30 function. The CPSF complex's interaction with apoptotic regulators like BCL2L11 suggests its potential role in conditions where apoptosis is dysregulated. Understanding these interactions may offer insights into the mechanistic pathways contributing to these diseases providing potential targets for therapeutic intervention.