Abcam, ab131259, Anti-Claudin 5 antibody [EPR7583]

Size: 20µL / 100µL / 1mL
Anti-Claudin 5 antibody [EPR7583] (ab131259) is a rabbit monoclonal antibody detecting Claudin 5 in Western Blot, IHC-P, ICC/IF . Suitable for Human . - Biophysical QC for unrivalled batch-batch consistency - Over 90 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR7583,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:ab131259 failed to detect target band in mouse brain tissue. This antibody is unsuitable to be used in IHC on mouse tissues.This antibody failed to get signal in cell lines when performing western blot, so we do not recommend to use it when testing cell lines in westen blot.

Product details:
Product Specifications
Anti-Claudin 5 antibody [EPR7583] (ab131259) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, WB in human samples.
Anti-Claudin 5 antibody [EPR7583] (ab131259) specifically detects Claudin 5 (UniProt ID: O00501; Molecular weight: 23kDa) and is sold in 100 µL and 1 mL selling sizes.
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Claudin 5 antibody [EPR7583] (ab131259) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Claudin 5 antibody [EPR7583] (ab131259) has been cited over 90 times in peer reviewed journals and is trusted by the scientific community.
Anti-Claudin 5 antibody [EPR7583] (ab131259) has 7 independent reviews from customers.
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Target Information
Claudin-5 (CLDN5) is an integral membrane protein that forms tight junctions in endothelial cells, crucial for the blood-brain barrier's (BBB) function. It ensures the selective permeability of the BBB, preventing harmful substances from entering the brain. Abnormal expression of Claudin-5 is associated with several neurological conditions, such as epilepsy, Alzheimer's disease, and schizophrenia, highlighting its importance in maintaining BBB integrity and brain health.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Claudin 5 also known as CLDN5 is an integral component of tight junction strands within endothelial and epithelial cells. This protein possesses a molecular weight of approximately 23 kDa. It prominently expresses in the vascular endothelial cells of the blood-brain barrier and other blood-tissue barriers. Claudin 5 plays a mechanical role in forming selectively permeable paracellular barriers that regulate the movement of molecules and ions between cells. This function is essential in preserving the tissue-specific environment.
Biological function summary
Claudin 5 serves as an important element in maintaining the integrity of the blood-brain barrier by creating a selective permeability. It operates as part of a larger tight junction complex interacting mainly with other claudin proteins and proteins such as occludin and ZO-1. These interactions ensure the structural and functional integrity of the cell junctions which helps stabilize the barrier properties against various physiological and pathological insults.
Pathways
Claudin 5 is involved in important biological pathways such as the tight junction pathway and the barrier permeability regulation pathway. Through these pathways it interacts with proteins like occludin and various signaling molecules. This involvement allows tight regulation of barrier permeability ensuring selective transport and preventing the passage of harmful substances into sensitive areas like the central nervous system.
Claudin 5 plays a significant role in conditions like cerebral edema and Alzheimer's disease. Altered expression or dysfunction of Claudin 5 disrupts the blood-brain barrier contributing to these disorders. In Alzheimer's disease there is evidence of impaired tight junctions where Claudin 5 interacts with other claudins and related proteins like amyloid-beta affecting barrier integrity. Understanding these connections helps in developing therapeutic strategies targeting tight junction stability.