Abcam, ab131368, Anti-mouse IgG for IP (HRP)

Size: 100µL
Rat anti-mouse IgG (gamma chain-specific) (HRP) (ab131368) a horseradish peroxidase-secondary antibody designed for sensitive chemiluminescent detection in Western blot, IP and ELISA applications. - Proven performance: cited in over 145 publications
Key facts
Host species:Rat,
Target species:Mouse,
Target isotype:IgG,
Target specificity:Gamma chain,
Pre-adsorbed:No,
Conjugation:HRP,
Applications:WB, ELISA, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Clonality:Monoclonal,
Clone number:eB144,
Isotype:IgG

Product details:
Number of blots:
At least
20 blots,
based on a 1 μl/ml dilution and 5 ml diluted antibody per blot.
Important protocol notes:
1. The anti-mouse IgG for IP secondary antibody (HRP) detects mouse IgG antibodies (subtypes: IgG1, IgG2a, IgG2b, IgG3).
2. The anti-mouse IgG for IP secondary antibody (HRP) preferentially detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) over the reduced, SDS-denatured forms.
3. IP sample should be completely reduced/denatured before loaded onto a western blot.
4. Milk should be used as the blocking protein for the immunoblot.
Western blot and IP resources:
Western blot a beginner's guide
IP protocol
IP troubleshooting tips

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.16% Sodium phosphate, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C