Abcam, ab131442, Anti-p53 antibody

Size: 100µg
Anti-p53 antibody (ab131442) is a rabbit polyclonal antibody detecting p53 in Western Blot, IHC-P . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Over 260 publications
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human TP53 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P04637

Product details:
Product Specifications
Anti-p53 antibody (ab131442) is a rabbit polyclonal antibody and is validated for use in IHC-P, WB in human, mouse, rat samples.
Anti-p53 antibody (ab131442) specifically detects p53 (UniProt ID: P04637; Molecular weight: 44kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-p53 antibody (ab131442) has high sensitivity and specificity.
The specificity of Anti-p53 antibody (ab131442) has been confirmed by testing in knockout samples.
Anti-p53 antibody (ab131442) has been cited over 266 times in peer reviewed journals and is trusted by the scientific community.
Anti-p53 antibody (ab131442) has 6 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-ab131442 was purified by affinity-chromatography using epitope-specific peptide., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, -20°C, Appropriate long-term storage conditions-+4°C, -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.
Biological function summary
P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.
Pathways
P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.
P53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.