Abcam, ab132106, Recombinant Human NR1D1 protein

Size: 10µg
Recombinant Human NR1D1 protein is a Human Full Length protein, in the 1 to 614 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:ELISA, WB, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P20393,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NR1D1 also known as Rev-Erbα is a nuclear receptor with a molecular weight of approximately 67 kDa. This protein acts as a transcriptional repressor and is a critical regulator of cyclic gene expression. NR1D1 binds to DNA and influences the transcription of genes by recruiting corepressor complexes reversing the activity of retinoic acid-related orphan receptors. It is expressed in various tissues including the liver adipose tissue and skeletal muscle highlighting its widespread significance in numerous biological processes.
Biological function summary
NR1D1 modulates the circadian rhythm and metabolic pathways. It integrates into the circadian complex synchronizing the expression of clock genes with metabolic signals. It regulates genes involved in lipid metabolism glucose homeostasis and the inflammatory response. Through its oscillatory gene regulation NR1D1 ensures that the physiological processes align with the day-night cycle affecting energy balance and lipid storage.
Pathways
NR1D1 functions significantly in the circadian rhythm and metabolism. It interacts with proteins like BMAL1 and CLOCK in the transcriptional feedback loop that generates circadian rhythms. Additionally NR1D1 involves itself in the regulation of the AMP-activated protein kinase (AMPK) pathway linking energy homeostasis to circadian regulation. These partnerships impact how the body manages energy during different phases of the day.
NR1D1 associates with metabolic diseases such as obesity and diabetes. Dysregulation of NR1D1 can contribute to metabolic syndrome by disrupting circadian timing and energy balance leading to compromised glucose and lipid metabolism. The protein also shows connections with inflammatory diseases given its role in modulating the inflammatory response. Aberrant NR1D1 activity can influence other proteins like NF-kB which is pivotal in inflammation and immune response pathways potentially exacerbating disease states.