Abcam, ab13219, Anti-CD11+CD18 antibody [24]

Size: 100µg
Mouse Monoclonal ITAL antibody. Suitable for ICC/IF, IP, Flow Cyt, WB, RIA and reacts with Human, Dog, Mouse, Rat samples. Cited in 28 publications. Immunogen corresponding to Cell preparation containing ITGB2 protein.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:24,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human, Dog, Rat, Mouse,
Applications:ICC/IF, Flow Cyt, WB, RIA, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Cell preparation containing ITGB2 protein. The exact immunogen used to generate this antibody is proprietary information.P05107,
Epitope:Recognises an epitope which is expressed by beta 2 (CD18) integrins in high affinity conformation.,
Specificity:This antibody specifically recognises the human CD11+CD18 activation epitope.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A/G, Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD11+CD18 also known as integrin αLβ2 or LFA-1 is a heterodimeric protein complex with a molecular weight of approximately 180 kDa. It consists of two subunits CD11a (αL) and CD18 (β2) which are expressed on the surface of leukocytes such as neutrophils monocytes and lymphocytes. This complex plays an important role in cell-cell adhesion and interactions with the extracellular matrix. CD11+CD18 is an essential mediator of leukocyte adhesion and is involved in the immune response.
Biological function summary
CD11a/CD18 enables leukocyte adhesion to endothelial cells and guides their migration towards sites of inflammation. It functions as part of a larger integrin family responsible for various cellular processes. The protein complex binds to its ligand ICAM-1 (Intercellular Adhesion Molecule 1) initiating a cascade of signaling events that facilitate immune surveillance and cell junction formation. This adhesion complex is integral to the regulation of immune system functions.
Pathways
CD11a and CD18 participate in the leukocyte extravasation pathway which is important for trafficking immune cells to areas of injury or infection. The protein complex also interacts within the T-cell receptor signaling pathway assisting in T-cell activation and proliferation. It works alongside proteins such as ICAM-1 to maintain cellular communication and adhesion allowing for a coordinated immune response. The involvement of CD11+CD18 in these pathways highlights its role in mediating inflammatory responses.
CD11+CD18 is associated with inflammatory diseases like rheumatoid arthritis and leukocyte adhesion deficiency (LAD). In rheumatoid arthritis the overexpression and dysregulation of this integrin can contribute to the chronic inflammation characteristic of the disease. In LAD mutations affecting CD18 hinder normal leukocyte adhesion leading to recurrent infections. Aberrations in the expression or function of CD18 can affect other proteins in the immune system linking it to various immune-related disorders and immune system dysregulation.