Abcam, ab132277, Recombinant Human N WASP protein

Size: 10µg
Recombinant Human N WASP protein is a Human Full Length protein, in the 1 to 505 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:WB, SDS-PAGE, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:O00401,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein N-WASP also known as neural Wiskott-Aldrich syndrome protein plays an important role in actin cytoskeleton dynamics. It weighs about 65 kDa and is widely expressed in diverse tissues but shows higher expression levels in the brain. In its mechanical function N-WASP regulates the polymerization of actin by activating the Arp2/3 complex. Through these interactions it helps in the formation of branched actin networks which are essential for cellular movement and shape.
Biological function summary
N-WASP functions are critical in cell signaling endocytosis and neurite extension. N-WASP often acts within a complex and interacts with various proteins like Cdc42 to transmit signals from cell surface to the actin cytoskeleton. These activities position N-WASP as a fundamental component for cellular architecture and mobility impacting processes like immune response and neuronal development. Its interaction with Arp2/3 contributes to dynamic changes in cell movement and shape.
Pathways
N-WASP plays a role in Rac and Cdc42 signaling pathways important for actin reorganization. Within these pathways it interacts with proteins such as Rho family GTPases influencing cytoskeletal arrangements. The proper functioning of these pathways is vital for numerous cellular processes including the migration of cells which affects tissue development and repair.
Alterations in N-WASP are linked to immunodeficiencies and neurological disorders. Mutations or dysfunction in N-WASP can lead to Wiskott-Aldrich syndrome characterized by immune defects and eczema due to its role in immune cell signaling. Additionally N-WASP's interaction with proteins such as WASP contributes to its involvement in neurodevelopmental disorders like autistic spectrum disorders reflecting the importance of this protein in maintaining normal cellular function.