Abcam, ab132512, Recombinant Human WIPF1 protein

Size: 10µg
Recombinant Human WIPF1 protein is a Human Full Length protein, in the 1 to 358 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:Tag free,
Applications:ELISA, SDS-PAGE, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:O43516,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The WIPF1 protein also known as WASP Interacting Protein or WIP plays a mechanical role in the regulation and stabilization of the actin cytoskeleton. It weighs approximately 65 kDa. Scientists find WIPF1 highly expressed in a variety of tissues including the hematopoietic cells where it influences diverse cellular activities. It maintains normal cellular functions by promoting actin polymerization and interacting with actin-binding proteins.
Biological function summary
The interaction of WIPF1 extends to facilitating cell morphology and movement. It acts as part of a complex by binding to WASP (Wiskott-Aldrich Syndrome Protein) which regulates the building of actin filaments in response to cellular signals. Through its biological actions WIPF1 contributes to processes such as endocytosis phagocytosis and cell migration. These roles are essential for maintaining cell dynamics and structure.
Pathways
The involvement of WIPF1 in the actin regulation pathways is significant. It participates in pathways like the Rho GTPase pathway which controls actin dynamics and the Arp2/3 complex-mediated pathway which promotes branching in actin filaments. The interaction of WIPF1 with proteins such as CDC42 and N-WASP highlights its importance in signal transduction leading to actin assembly and cellular responses.
Mutations or dysfunctions in WIPF1 associate with certain immunodeficiencies such as Wiskott-Aldrich syndrome where defective actin regulation occurs. Aberrations in WIPF1 expression also have associations with cancers particularly those affecting hematopoietic cells. The protein's link with WASP underlines its role in these conditions highlighting the importance of proper actin dynamics for normal immune function and cellular stability.