Abcam, ab13252, Anti-Rab4 antibody - Early Endosome Marker

Size: 200µg
Rabbit Polyclonal Rab4 antibody. Early Endosome marker. Suitable for ICC, WB and reacts with Rat samples. Cited in 42 publications. Immunogen corresponding to Recombinant Protein within Human RAB4A.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat,
Applications:ICC, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Protein within Human RAB4A. The exact immunogen used to generate this antibody is proprietary information.P20338,
Specificity:This antibody is very specific for Rab4. To our knowledge this antibody does not cross-react with other Rab proteins. However, as there are dozens of different proteins in the Rab family, not every single one has been tested for cross-reactivity with this antibody.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rab4 also known as Ras-related protein Rab-4A is a small GTPase with a molecular mass of approximately 24 kDa. This protein is expressed in various cell types predominantly in tissues with high endocytic activity. Rab4 primarily functions as an endosome marker cooperating with the endocytic machinery to regulate sorting and recycling of endosomes. It occupies key positions on early endosomes relevant to its role in vesicular trafficking and membrane transportation. Endosome staining typically identifies Rab4 as an essential component in understanding endocytosis dynamics.
Biological function summary
Rab4 coordinates the trafficking of cargo between endosomes and the plasma membrane. It usually forms part of a protein complex that governs the recycling pathway of internalized membrane proteins and lipids. Rab4 modulates various cellular processes by impacting the rapid recycling of receptors receptors often return to the cell surface in response to Rab4 regulation. This action is important for maintaining cellular homeostasis and efficient signal transduction serving as a checkpoint for receptor-mediated events.
Pathways
The protein plays significant roles in the endocytic recycling pathway and the insulin signaling pathway. Rab4's activity influences the trafficking cycle often interacting with other proteins such as Rab11 and Rab5 to ensure efficient cargo movement within cells. By facilitating swift endosome recycling Rab4 has a direct impact on intracellular signaling and trafficking pathways supporting a range of cellular activities. This concerted interaction accentuates its importance in managing endosomal traffic and maintaining cellular connectivity.
Rab4 has implications in neurodegenerative disease and cancer. Abnormal Rab4 function disrupts endosomal signaling contributing to the pathogenesis of conditions like Alzheimer’s disease where it interacts with proteins such as amyloid precursor protein (APP). In cancer the dysregulation of Rab4-associated pathways affects tumor progression and metastasis. Rab4's connection with proteins like EGFR (Epidermal Growth Factor Receptor) indicates its potential role in altering cancer cell behaviors and resistance mechanisms making it a focus of interest in therapeutic approaches.