Abcam, ab132557, Recombinant Human Abhd5/CGI-58 protein

Size: 10µg
Recombinant Human Abhd5/CGI-58 protein is a Human Full Length protein, in the 1 to 349 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:ELISA, WB, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q8WTS1,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione

Product details:
This product was previously labelled as Abhd5.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Abhd5 also known as CGI-58 is a protein with significant importance in lipid metabolism. It has a molecular weight of approximately 39 kDa and is ubiquitously expressed in tissues throughout the body including adipose tissue and liver. This protein associates with lipid droplets within cells serving a role important for lipid handling and signaling. Abhd5 stands for the alpha/beta hydrolase domain-containing protein 5 indicative of its structure and enzymatic functions.
Biological function summary
The Abhd5/CGI-58 protein acts as a coactivator of adipose triglyceride lipase (ATGL) enhancing its role in triglyceride hydrolysis. This support is vital for energy release from triglycerides in fat cells. Abhd5 does not function alone; it exists within a protein complex where it modulates ATGL's catalytic activity. Furthermore Abhd5 is implicated in broader lipid signaling and metabolic control influencing various cellular responses through its roles within these complexes.
Pathways
Abhd5/CGI-58 is central to the lipid metabolism pathway particularly in the breakdown of triglycerides. By activating ATGL Abhd5 influences the lipolytic pathway which is important for mobilizing stored fats for energy use. In addition to its role with ATGL another protein perilipin regulates this pathway by interacting with lipid droplet-associated proteins including Abhd5 to control access to lipids. The protein influences the signaling crosstalk within metabolic pathways coordinating metabolic responses within the cell.
The Abhd5/CGI-58 protein has ties to conditions like lipodystrophy and Chanarin-Dorfman syndrome. Deficiencies or mutations in the CGI-58 protein can impair lipid metabolism leading to abnormal fat distribution and storage. In these diseases the lack of Abhd5 activity affects the interactions with proteins like ATGL which is responsible for triglyceride breakdown. Research continues to explore the relationship between Abhd5 dysfunction and these metabolic disorders aiming to clarify its role in the etiology of such conditions.