Abcam, ab133040, Leukotriene B4 ELISA Kit

Size: 1 x 96Tests
Leukotriene B4 ELISA Kit is a Competitive ELISA for the measurement of Leukotriene B4 in Human, Pig in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Saliva, Urine, Plasma, Cell culture supernatant,
Reacts with:Human, Pig,
Assay type:Competitive,
Sensitivity:= 5.63 pg/mL,
Range:11.7 - 3000 pg/mL,
Assay time:4h,
Assay Platform:Microplate

Product details:
Abcam's Leukotriene B
in vitro
competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Leukotriene B
) in tissue culture media, plasma, urine and saliva.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-LTB
antigen and a polyclonal rabbit antibody specific to LTB
. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of LTB
captured in the plate.
Leukotrienes are major products of 5-lipoxygenase metabolism of arachidonic acid. Leukotriene B
) stimulates leukocyte functions including lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB
has been implicated as a potent mediator of inflammatory diseases and immunoregulation. The cysteinyl leukotrienes (LTC
, and LTE
) are formed by addition of cysteine derivatives to LTA
. The conversion of LTA
to LTC
by the enzyme LTC
synthase limits the rate of cysteinyl leukotriene formation. Cysteinyl leukotrienes are potent lipid mediators in inflammation, inducing smooth muscle contractions and increased capillary permeability.
Cross Reactivity
Compound
Cross Reactivity %
6-trans-12-epi-LTB
12-epi-LTB
20-OH-LTB
20-COOH-LTB
5(S)-HETE
12(S)-HETE
15(S)-HETE

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Leukotriene B4 commonly referred to as LTB4 is a bioactive lipid molecule with an approximate molecular mass of 279.45 g/mol. It is a part of the leukotriene family which are eicosanoids synthesized from arachidonic acid via the 5-lipoxygenase (5-LO) pathway. LTB4 is primarily expressed by leukocytes such as neutrophils as well as macrophages and mast cells. Its production occurs mostly in response to inflammatory stimuli which makes it an important mediator in immune responses.
Biological function summary
LTB4 acts as a potent chemoattractant and activator of leukocytes mediating immune cell recruitment to sites of inflammation. It binds specifically to G-protein-coupled receptors BLT1 and BLT2 on the surface of target cells leading to activation and subsequent response. LTB4 is not part of a larger protein complex; instead it functions as a standalone signaling lipid. Through its interaction with BLT receptors it plays a role in amplifying inflammatory signals contributing to the body's defense mechanisms.
Pathways
LTB4 is deeply embedded in the arachidonic acid metabolic pathway which includes the lipoxygenase and cyclooxygenase paths. This pathway is essential for the production of other eicosanoids involved in inflammation and immunity such as prostaglandins. LTB4 works alongside other leukotrienes in modulating the inflammatory response. It is closely related to proteins like 5-lipoxygenase which catalyzes the initial steps leading to its synthesis and also interacts with protein kinase pathways that mediate cellular responses.
LTB4 has significant relevance in chronic inflammatory conditions like asthma and rheumatoid arthritis. In asthma it contributes to bronchoconstriction and leukocyte infiltration which worsen the disease symptoms. Rheumatoid arthritis sees LTB4 facilitating the infiltration and activation of immune cells within joints thereby exacerbating inflammation and tissue damage. It connects to proteins like interleukin-8 (IL-8) in these contexts which further propagate inflammatory signals highlighting LTB4's critical role in the pathology of inflammatory diseases.