Abcam, ab133045, Mouse IgG1 ELISA Kit

Size: 1 x 96Tests
Mouse IgG1 ELISA Kit is a sandwich ELISA designed to quantify Mouse IgG1 with a sensitivity of 0.116 ng/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 7.81 - 250 ng/mL
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.116 ng/mL,
Range:7.81 - 250 ng/mL,
Assay time:90m,
Assay Platform:Microplate

Product details:
Mouse IgG1 ELISA Kit ab133045 is a sandwich ELISA to measure Mouse IgG1 in serum, cell culture supernatant with a sensitivity of 0.116 ng/ml.
How the assay works
specific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated IgG
detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a colored product that changes after adding acidic stop solution. The density of coloration is directly proportional to the IgG
amount of sample captured in plate.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Mouse IgG1 ELISA Kit ab133045 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature
4. Aspirate and wash each well.
5. Add TMB Substrate Solution to each well.
6. Immediately begin recording the color development
IgG is divided into four subclasses; IgG1, IgG2, IgG3, and IgG4. IgG
is the most abundant immunoglobulin found in the blood. It is a glycoprotein which consists of two identical heavy chains (50 kDa each) and two identical light chains (25 kDa each), to give a combined mass of approximately 150 kDa. The chains are held in place by covalent disulfide bonds. Each light chain contains two immunoglobulin (Ig) domains, while the heavy chains contain four Ig domains each. In the middle of each heavy chain is a relative varying portion called the "hinge region" which is unique to each IgG. This region allows for molecular flexibility and sets IgG
apart from its IgG counterparts. IgG1 properties and functions include neutralization, opsonization, activation of the complement system, diffusion into extravascular sites and crossing the placenta.
Cross Reactivity
Compound
Cross Reactivity
Rat IgG1
Mouse IgG2b
Human IgG1
<0.01%
Mouse IgG2a
<0.01%
Mouse IgG3
<0.01%
Mouse IgM
<0.01%
Recommended dilution of serum is 1:20,000

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols