Abcam, ab133053, Serotonin ELISA Kit

Size: 1 x 96Tests
The Serotonin (5-HT) ELISA kit (colorimetric) is a competitive ELISA designed to quantify serotonin (5-hydroxytryptamine, 5-HT) in serum, plasma, urine, and platelets with a sensitivity of 0.293 ng/mL.
Key facts
Detection method:Colorimetric,
Sample types:Urine, Plasma, Serum, Platelets,
Assay type:Competitive,
Sensitivity:= 0.293 ng/mL,
Range:0.49 - 500 ng/mL,
Assay time:3h,
Assay Platform:Microplate

Product details:
Serotonin ELISA Kit ab133053 is a competitive ELISA to measure Serotonin (5 hydroxytryptamine) in Urine, Plasma, Serum, Platelets with a sensitivity of 0.293 ng/mL.
How the assay works
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated- Serotonin antigen and a polyclonal rabbit antibody specific to Serotonin. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Serotonin captured in the plate.
Assay specificity
Cross reactivity:
- N-Acetyl Serotonin : 17%
- 5-Hydroxy-L-tryptophan: 0.4%
- Tryptamine: 0.1%
- 5-Hydroxyindoleacetic acid: 0.03%
- Melatonin: 0.01%
- Tyramine: <0.004%
- Tryptophan: <0.004%
Serotonin ELISA Kit ab133053 protocol summary
1. Add samples and standards to the wells
2. Add prepared labeled AP-conjugate to appropriate wells
3. Add Serotonin antibody to appropriate wells. Incubate at room temperature
4. Add pNpp substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read immediately
How other researchers are using Serotonin ELISA kit ab133053
Serotonin ELISA kit ab133053 has been used in publications to study the role of peripheral MSCs in major depressive disorder
, rehabilitation effects on inflammation and sarcopenia
, and the correlation between seratonin levels and heart failure
References:
Andy Peng Xiang et al. 2023 PMID: 37973914,
Mario Clerici et al. 2022 PMID: 36010554,
Roselyn Barbara Rose'Meyer et al. 2020 PMID: 34645867
Related and recommended products
Serotonin ELISA kit ab133053 is commonly used to study behavioral and neurodegenerative disoders. Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine found in the central nervous system, gastrointestinal tract, and blood with broad physiological functions as a neurotransmitter, in gastric motility, hemostasis, and cardiovascular integrity. Defects in serotonin signaling have been linked to a large number of complex behavioral disorders in humans, including anxiety and depression, autism, and eating disorders, and to neurodegenerative disorders such as Parkinson's. Platelets serve as the major reservoir of serotonin in the bloodstream. When activated, platelets release serotonin into the bloodstream where it acts as a powerful vasoconstrictor. Treatment with Selective Serotonin Uptake Inhibitors (SSRIs) dramatically reduces platelet serotonin concentrations, and altered levels of serotonin in the circulatory system are implicated in such diverse conditions such as asthma, liver cirrhosis and carcinoid tumors.
Other kits for measuring neurotransmitters are:
- Dopamine ELISA Kit
ab285238
- Glutamate Assay Kit
ab83389
- Norepinephrine ELISA Kit
ab287789
- Adrenaline ELISA Kit
ab287788
- Human Acetylcholine ELISA Kit
ab287811
- Human GABA ELISA Kit
ab287792
Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine found in the central nervous system, gastrointestinal tract, and blood with broad physiological functions as a neurotransmitter, in gastric motility, hemostasis, and cardiovascular integrity. Defects in serotonin signaling have been linked to a large number of complex behavioral disorders in humans, including anxiety and depression, autism, and eating disorders, and to neurodegenerative disorders such as Parkinson's. Platelets serve as the major reservoir of serotonin in the bloodstream. When activated, platelets release serotonin into the bloodstream where it acts as a powerful vasoconstrictor. Treatment with Selective Serotonin Uptake Inhibitors (SSRIs) dramatically reduces platelet serotonin concentrations, and altered levels of serotonin in the circulatory system are implicated in such diverse conditions such as asthma, liver cirrhosis and carcinoid tumors.
Cross reactivities:
Compound
Cross Reactivity
N-Acetyl Serotonin
5-Hydroxy-L-tryptophan
Tryptamine
5-Hydroxyindoleacetic acid
Melatonin
Tyramine
<0.004%
Tryptophan
<0.004%

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Serotonin also known as 5-hydroxytryptamine (5-HT) is a neurotransmitter mainly involved in modulating mood emotion and behavior. It weighs approximately 176.2 Da and appears in several body tissues including the central nervous system gastrointestinal tract and blood platelets. Neurons within the raphe nuclei of the brainstem predominantly synthesize serotonin influencing various physiological processes. Its mechanical role involves binding to and activating specific serotonin receptors classified into seven different types each mediating distinct signaling pathways.
Biological function summary
Serotonin regulates multiple bodily functions beyond mood such as appetite sleep and circadian rhythm. It forms part of a complex system interacting with various receptor subtypes which include the anti-serotonergic pathways inhibiting its action. Each receptor subtype leads to different downstream effects in cells contributing to the diversity of serotonin's biological roles. For research purposes scientists often use an ELISA kit to measure serotonin levels and this can influence the ELISA test cost based on the specificity and sensitivity required.
Pathways
Serotonin participates in both the serotonergic and dopaminergic pathways illustrating its central role in neurotransmission. The serotonergic pathway is essential for transmitting nerve impulses while its interaction with the dopaminergic system significantly affects mood and reward-related behaviors. Key proteins such as tryptophan hydroxylase for serotonin synthesis and the vesicular monoamine transporter are involved within these pathways and may alter serotonin's functional availability.
Serotonin is closely linked to depression and anxiety disorders where its imbalance can lead to mood disturbances. Antidepressant treatments often target serotonin receptors or its transporters restoring its levels within synapses. Serotonin's connection to these conditions extends through its relationship with proteins like the serotonin transporter (SERT) which recycles serotonin in the synaptic cleft. Understanding these dynamics presents potential targets for interventions and anti-serotonergic agents may offer therapeutic options for managing such disorders.