Abcam, ab133102, Adipogenesis Assay Kit (Cell-Based)

Size: 1Kit
Adipogenesis Assay Kit (ab133102) provides the reagents required for studying the induction and inhibition of adipogenesis in the established 3T3-L1 model using the adipogenesis induction procedure.
Key facts
Detection method:Colorimetric,
Sample types:Adherent cells,
Assay type:Cell-based,
Assay Platform:Microplate reader

Product details:
Adipogenesis Assay Kit (ab133102) provides the reagents required for studying the induction and inhibition of adipogenesis in the established 3T3-L1 model using the adipogenesis induction procedure. This kit can also be used to screen drug candidates involved in adipogenesis. The classic Oil Red O staining for lipid droplets is used in this kit as an indicator of the degree of adipogenesis and can be quantified with a plate reader after the dye is conveniently extracted from the lipid droplet.
Obesity is a risk factor in many of the major chronic diseases such as diabetes mellitus, and cancer. The ability to regulate the number of fat cells and/or the size of fat cells is a key in the development and physiology of obesity and also in the origin of chronic disease. Mammals have both brown adipose tissue (BAT), which utilizes lipids to generate heat in a process known as thermogenesis, and white adipose tissue (WAT) which stores excess energy as triglycerides in lipid droplets. Adipocytes are derived from multipotent mesenchymal precursor cells commit to preadipocytes and then either remain dormant or proceed to become differentiated adipocytes.
3T3-L1 cells are a well-characterized cell line to study the differentiation of adipocytes, and other mechanisms such as insulin-induced glucose uptake and mechanisms of obesity development. This model system has greatly advanced the understanding of the molecular basis and signaling pathways of
adipogenesis
. During terminal differentiation, the fibroblast-like preadipocytes undergo a series of morphological and biochemical changes to eventually accumulate lipid droplets. This
in vitro
differentitated adipocytes share similar morphology with adipocytes
in vivo

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Adipogenesis also known as fat cell differentiation refers to the process of creating mature adipocytes from precursor cells such as mesenchymal stem cells. The process involves different stages marked by the expression of several key transcription factors like C/EBPα and PPARγ. These factors regulate the conversion of preadipocytes into fully functional adipocytes. Primary sites for adipogenesis include white and brown adipose tissues and this process can be studied using various detection methods including fluorometric detection and adipored assays.
Biological function summary
The formation of adipocytes involves the activation of specific gene networks that govern lipid accumulation and insulin sensitivity. Adipogenesis plays a significant role in energy storage and homeostasis by having the function of sequestering lipids in the form of triglycerides. It involves complex signaling pathways where multiple proteins interact and coordinate to facilitate lipid droplet formation and adipocyte maturation.
Pathways
Adipocyte development is tightly integrated into the insulin signaling and lipid metabolism pathways. Within these pathways adipogenesis interacts with proteins like AKT and mTOR which are important for promoting lipid accumulation and maintaining energy balance. The pathways form a coordinated system that ensures efficient energy storage and mobilization depending on metabolic demands.
Improper regulation of adipogenesis is linked with metabolic diseases such as obesity and type 2 diabetes. Dysregulated adipocyte formation can contribute to ectopic lipid accumulation and insulin resistance. The process of adipogenesis influences pathological states through proteins such as TNF-α which is elevated in these disorders and negatively impacts adipocyte function. Proper understanding and manipulation of this process offer potential therapeutic avenues for controlling obesity-related complications.