Abcam, ab133406, ECL Substrate Kit (High Sensitivity)

Size: 20mL / 200mL / 500mL
ECL Substrate Kit (High Sensitivity) (ab133406) is an enhanced chemiluminescent substrate designed for the detection of proteins with 23 pg-187 ng of protein per band. - Long lasting, highly sensitivity substrate- strong signal with very low background - Use for film imaging and compatible with CCD imaging - Available in different sizes to fit your experimental needs
Product details:
High Sensitivity ECL Substrate Kit ab133406 is designed for the detection of proteins with 23pg-187ng of protein per band. It uses an enhanced chemiluminescent substrate for western blotting that was developed for film imaging and is also compatible with CCD imaging. The high sensitivity ECL substrate produces a strong signal with very low background. Additionally, the ECL signal is long lasting, allowing repeated exposures without fear of losing data.
Our ECL kits include our popular ECL Substrate Kit
ab65623
and our high sensitivity ECL substrate kits:
- this kit (High Sensitivity ECL Substrate Kit ab133406) to detect 23pg-187ng of protein per band
- Very High Sensitivity ECL Substrate Kit
ab133408
to detect 4.6pg-4.7ng of protein per band
- Ultra High Sensitivity ECL Substrate Kit
ab133409
to detect 1.2pg-2ng of protein per band
Detection ranges in pg and ng stated above should be used for guidance only as detection range is dependent on the molecular weight of a protein.
This product was previously called Optiblot ECL Detect Kit (23pg-187ng).
- 200ml kit for 2000cm
membrane
- 500ml kit for 5000cm
membrane
The primary antibody can often be diluted 5 to 10 fold more than usual when using this ECL substrate. A typical primary antibody dilution range using the substrate is 1/5000 - 1/20,000, with a typical secondary antibody dilution range of 1/20,000 - 1/100,000. Some optimisation may be required.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C