Abcam, ab133408, ECL Substrate Kit (Very High Sensitivity)

Size: 20mL / 100mL / 200mL
Very High Sensitivity ECL Substrate Kit () ab133408 uses a new horseradish peroxidase (HRP) substrate specially developed to take advantage of the large linear dynamic range provided by CCD imaging.
Product details:
Very High Sensitivity ECL Substrate Kit () ab133408 uses a new horseradish peroxidase (HRP) substrate specially developed to take advantage of the large linear dynamic range provided by CCD imaging. It is designed for the detection of proteins from 4.6pg to 4.7ng of protein per band.
ECL Substrate Kit ab133408 produces a strong, extremely long-lived signal, which, combined with very low background levels, allows for long exposure times enabling the detection of low-abundance proteins. Additionally, the signal from the ECL substrate is linear with respect to protein amount over a broad range of concentrations, displaying no substrate depletion at high protein loads, allowing the user to take full advantage of the linear range of the CCD detection method.
The ECL substrate is also compatible with X-ray film detection, though the limited dynamic range of film will make resulting data less quantitative.
Our ECL kits include our popular ECL Substrate Kit
ab65623
and our high sensitivity ECL substrate kits:
- High Sensitivity ECL Substrate Kit
ab133406
to detect 23pg-187ng of protein per band
- this kit (Very High Sensitivity ECL Substrate Kit ab133408) to detect 4.6pg-4.7ng of protein per band
- Ultra High Sensitivity ECL Substrate Kit
ab133409
to detect 1.2pg-2ng of protein per band
Detection ranges in pg and ng stated above should be used for guidance only as detection range is dependent on the molecular weight of a protein.
This product was previously called Optiblot ECL Max Detect Kit (4.6pg-4.7ng).
- 100ml kit for 1000cm
membrane
- 200ml kit for 2000cm
membrane
The primary antibody can often be diluted 5 to 10 fold more than usual when using this ECL substrate. A typical primary antibody dilution range using the substrate is 1/5000 - 1/20,000, with a typical secondary antibody dilution range of 1/20,000 - 1/100,000. Some optimisation may be required.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C