Abcam, ab133592, Anti-Glutathione Synthetase antibody [EPR6563]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal Glutathione Synthetase antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 12 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR6563,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glutathione Synthetase (GSS) sometimes called GSS line is an enzyme that plays a central role in cellular function by catalyzing the ATP-dependent conversion of gamma-glutamylcysteine and glycine into glutathione. This enzyme has a molecular mass of about 70 kDa and exhibits high expression in the liver and red blood cells. It maintains the necessary cellular levels of glutathione a major antioxidant that protects cells from oxidative stress.
Biological function summary
GSS is responsible for the final step in glutathione biosynthesis. Acting independently GSS operates without forming a larger enzyme complex. Its enzymatic activity ensures adequate production of glutathione which regulates cellular redox homeostasis and detoxification processes. The availability of glutathione is critical for cellular respiration and immunity molecules essential for a well-functioning organism.
Pathways
The activity of GSS integrates into the gamma-glutamyl cycle and the detoxification pathway. It partners indirectly with enzymes like gamma-glutamylcysteine synthetase which precedes GSS in the glutathione biosynthesis pathway. This relationship positions GSS as an important element in sustaining normal cellular detoxification processes ensuring the neutralization of reactive oxygen species and maintaining the balance of free radicals.
Defects in GSS activity correlate with glutathione synthetase deficiency an autosomal recessive disorder that results in increased oxidative damage and metabolic acidosis. Symptoms range from hemolytic anemia to neurological complications due to impairments in oxidative stress management. The disorder links to proteins involved in antioxidant processes highlighting the importance of GSS in maintaining proper cellular function and disease prevention.