Abcam, ab133639, Anti-HFE antibody [EPR6751(2)]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal HFE antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR6751(2),
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
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Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The HFE protein also known as the hemochromatosis protein or HFE 5000 is a critical component in the regulation of iron absorption in the body. Structurally this protein has a known mass of about 50 kDa and is expressed primarily in the liver pancreas and intestines. The HFE belongs to the class I major histocompatibility complex (MHC) proteins. Its main function is to interact with transferrin receptors regulating the amount of iron that's absorbed from the diet into the bloodstream.
Biological function summary
This protein plays a significant role in maintaining iron homeostasis within the body. HFE does not function alone; it forms a complex with transferrin receptors to modulate iron uptake. Its interaction with the transferrin-bound iron helps prevent excess iron build-up by reducing the binding affinity of transferrin for its receptor. Through these interactions HFE balances iron storage and transport ensuring proper iron levels in vital organs.
Pathways
HFE is an integral part of iron metabolism and homeostasis pathways. It closely interacts with proteins like TFR1 (Transferrin Receptor 1) in these pathways. The hepcidin pathway also involves HFE where it indirectly influences the synthesis of hepcidin by the liver. Hepcidin plays an important role in preventing iron overload by binding and degrading the iron export protein ferroportin hence regulating the release of iron from cells adding a layer of control over iron homeostasis.
HFE mutations can lead to hereditary hemochromatosis a condition characterized by excessive iron accumulation in tissues which leads to organ damage. A common mutation is the C282Y mutation which disrupts the protein's normal function. This disorder links to other proteins related to iron metabolism such as hepcidin. Proper functioning of HFE is essential to avoid diseases like hemochromatosis as its impairment results in misregulated iron levels leading to adverse health effects.