Abcam, ab134047, Anti-VCAM1 antibody [EPR5047]

Size: 100µL / 1mL
Anti-VCAM1 antibody [EPR5047] (ab134047) is a rabbit monoclonal antibody detecting VCAM1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF, ELISA . Suitable for Human, Mouse, Rat . - Clone EPR5047 is the most cited clone to VCAM1 - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 300 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5047,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WB, ICC/IF, Flow Cyt (Intra), IP, I-ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-VCAM1 antibody [EPR5047] (ab134047) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), I-ELISA, ICC/IF, IHC-P, IP and WB.
Anti-VCAM1 antibody [EPR5047] (ab134047) was first used in a scientific publication in 2013 and has been cited over 309 times in peer reviewed journals. It's performance in Western Blot and IHC in mouse and human samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-VCAM1 antibody [EPR5047] (ab134047) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-VCAM1 antibody [EPR5047] (ab134047) has been confirmed by Western Blot testing in VCAM1 knockout A549 cells (
ab273758
Anti-VCAM1 antibody [EPR5047] (ab134047) has 16 independent reviews from customers.
Anti-VCAM1 antibody [EPR5047] (ab134047) specifically detects VCAM1 (UniProt ID: P29533; Molecular weight: 79kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EPR5047 -
ab215380
Antibody clone EPR5047 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
647, HRP, PE, FITC, APC (
ab194319
ab195540
ab223981
ab223982
ab223983
- Specificity confirmed with SYP knockout cell line validation
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.
Biological function summary
Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.
Pathways
VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-κB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.
Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.