Abcam, ab134166, Anti-Slit2 antibody [EPR2771]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal SLIT2 antibody. Suitable for IHC-P, WB and reacts with Human, Transfected cell line - Human samples. Cited in 26 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR2771,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Slit2 is a secretory protein, so Brefeldin A (BFA) would help to increase the detection of Slit2 in cell lysate.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Slit2 also known as Slit homolog 2 protein is a guidance cue protein with a mass of approximately 200 kDa. It plays a role in axonal guidance and cellular migration. Slit2 is expressed in the central nervous system where it contributes to neural development. It is also found in various tissues such as the liver lungs and kidneys. Slit2 mediates its effects primarily through interaction with Roundabout (Robo) receptors.
Biological function summary
Slit2 functions in guiding axons during neuronal development and preventing them from straying from their pathways. It complexes with Robo receptors to transmit repulsive directional signals. This interaction is essential in the development of the nervous system ensuring proper neural circuit formation and synaptic connectivity. Besides neural guidance Slit2 also regulates other cellular events like angiogenesis and immune cell migration.
Pathways
Slit2 participates in the axon guidance pathway where it influences neural network structuring by interacting with Robo receptors and other guidance cues. The Slit-Robo pathway plays an important role in axonal repulsion working alongside the Netrin signaling pathway that involves proteins like DCC and UNC5. This way Slit2 and its pathways balance attractive and repulsive cues to form functional neuronal networks.
Slit2 has associations with cancers particularly glioblastoma where it affects tumor cell migration and invasion. Dysregulation of the Slit-Robo signaling may lead to enhanced cancer cell metastasis. Additionally Slit2 is linked to congenital heart diseases where its malfunctioning affects cardiac development. Its interaction with Robo and other proteins like Ephrin can influence these disease processes suggesting a broader role in both developmental and pathological contexts.