Abcam, ab134876, Recombinant human Oct4 protein

Size: 50µg
Recombinant human Oct4 protein is a Human Full Length protein, in the 1 to 360 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:Tag free,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Activity validated for generating iPS cells from human fibroblasts with 3 retroviral transduction (KLF4, SOX2 and cMyc),
Accession:Q01860,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 10% L-Arginine hydrochloride, 10% Sodium chloride, 2% Tris HCl, 1% Potassium chloride, 0.01% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.01% EDTA

Product details:
The protein is fusioned with a viral herpes simplex virus protein VP16 transactivation domain at its N-terminal and 11R tag at C-terminal end.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Oct4 also known as POU5F1 Oct 4 protein and hecho en Oct 4 is a transcription factor important for maintaining pluripotency in stem cells. Oct4 protein has a molecular weight of approximately 40-45 kDa. Stem and germ cells express Oct4 where it regulates gene expression essential for cell differentiation and self-renewal. In immunohistochemistry (IHC) studies scientists often use Oct4 staining to identify stem cell presence and behavior.
Biological function summary
Oct4 acts in concert with other pluripotency factors like SOX2 and NANOG to form a regulatory network essential for maintaining stem cell identity. As part of this network the Oct4 transcription factor orchestrates the expression of genes that prevent differentiation and maintain the stem cell’s undifferentiated state. The protein directly activates the transcription of target genes involved in maintaining the pluripotent state.
Pathways
Oct4 plays an important role in the maintenance of pluripotency and self-renewal in the embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) pathways. Oct4 partners with proteins such as SOX2 and KLF4 in these pathways to activate and repress a common set of downstream genes which control differentiation and proliferation. Oct4 ensures the blocking of differentiation signals while promoting self-renewal in pluripotent cells.
Aberrant Oct4 expression often links to oncogenesis and tumor progression particularly in testicular germ cell tumors and certain types of cancers. Oct4 overexpression may interact with c-MYC a protein linked to cancer cell proliferation advancing tumorigenesis. Additionally researchers investigate Oct4 as a diagnostic marker for cancer and its potential as a therapeutic target to inhibit cancer cell growth.