Abcam, ab13498, Anti-Superoxide Dismutase 1 antibody

Size: 50µg
Anti-Superoxide Dismutase 1 antibody (ab13498) is a rabbit polyclonal antibody for Western Blot, IP, IHC-P, IHC-Fr, ICC/IF, ELISA . Suitable for Coral, Cow, Dog, Drosophila, Fish, Hamster, Human, Mollusk, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Xenopus . - Over 190 publications - Trusted since 2004
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Monkey, Xenopus laevis, Cow, Fish, Coral, Pig, African green monkey, Sheep, Mouse, Hamster, Drosophila melanogaster, Mollusk, Rabbit, Dog, Rat,
Applications:IP, WB, ELISA, IHC-Fr, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human SOD1. The exact immunogen used to generate this antibody is proprietary information.P00441

Product details:
What is this antibody validated in?
Anti-Superoxide Dismutase 1 antibody (ab13498) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in African green monkey, Coral, Cow, Dog, Drosophila melanogaster, Fish, Hamster, Human, Mollusk, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Xenopus laevis samples.
What is the molecular weight of Superoxide Dismutase 1?
Anti-Superoxide Dismutase 1 (ab13498) specifically detects a band for Superoxide Dismutase 1 (UniProt: P00441) at a molecular weight of 16kDa.
Trusted by the scientific community
Anti-Superoxide Dismutase 1 (ab13498) was first used in a scientific publication in 2004 and has been cited over 190 times in peer-reviewed journals.
Reviewed by scientists
Anti-Superoxide Dismutase 1 (ab13498) has over 20 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-This antibody was purified on an antigen coupled sepharose column., Storage buffer-Preservative: 0.1% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Superoxide Dismutase 1 (SOD1) also known as Cu/Zn SOD or simply dismutase is an enzyme important for the detoxification of superoxide radicals. SOD1 catalyzes the conversion of two superoxide molecules into oxygen and hydrogen peroxide maintaining cellular redox balance. This enzyme typically exhibits a mass of approximately 32 kDa. SOD1 is expressed in the cytoplasm of cells throughout the body including tissues like liver kidney and brain.
Biological function summary
The enzyme functions as a homodimer with each subunit containing a copper and zinc ion. These metal ions are essential for the catalytic activity of SOD1 as the copper ion participates in electron transfer while the zinc ion provides structural stability. The enzyme protects cells from oxidative stress by neutralizing excess reactive oxygen species ensuring cellular health and functioning.
Pathways
SOD1 plays an important role in the cellular antioxidant defense system and is a part of the reactive oxygen species (ROS) metabolic pathway. It works in conjunction with catalase and glutathione peroxidase to limit oxidative damage within cells. The close interaction between these enzymes highlights the interdependence within the antioxidant defense network emphasizing their role in maintaining cellular homeostasis.
Mutations in the SOD1 gene are linked to familial Amyotrophic Lateral Sclerosis (ALS) a neurodegenerative condition. In ALS aberrant SOD1 proteins may lead to increased oxidative stress and motor neuron damage. Research also connects SOD1 to the pathogenesis of Alzheimer's disease where oxidative stress is a contributing factor. These associations underline SOD1's significance in neurodegenerative diseases highlighting potential therapeutic targets for interventions focused on oxidative stress management.