Abcam, ab135223, Recombinant Human INCENP protein

Size: 20µg / 50µg
Recombinant Human INCENP protein is a Human Fragment protein, in the 783 to 918 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB, FuncS.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:His tag N-Terminus,
Applications:FuncS, SDS-PAGE, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q9NQS7,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7Preservative: 1.02% ImidazoleConstituents: 25% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
INCENP short for Inner Centromere Protein plays a mechanical role during cell division. It acts as an important component of the chromosomal passenger complex (CPC) an important player in mitosis. INCENP is approximately 95 kDa in mass and is expressed largely in dividing cells. It helps to regulate chromosome alignment segregation and cytokinesis by interacting with other CPC members. Its activity is centered on the centromeres and the spindle midzone making it essential for proper mitotic progression.
Biological function summary
INCENP functions within the CPC which includes the kinase Aurora B Survivin and Borealin. This complex facilitates proper chromosomal alignment and segregation during mitosis. INCENP links Aurora B kinase activity to the centromere essential for correct chromosome bi-orientation and tension sensing. As a result INCENP stabilizes the architecture of the mitotic spindle ensuring successful cell cycle completion. This process is vital for maintaining genomic stability in proliferating cells.
Pathways
INCENP participates in the signaling cascades that control mitosis and cellular division. It is involved in the spindle assembly checkpoint pathway ensuring that cells do not progress through mitosis with misaligned chromosomes. INCENP interacts closely with proteins such as Aurora B and Survivin within this checkpoint mechanism. These interactions ensure that the cell cycle does not proceed until all chromosomes are correctly attached to the spindle apparatus thereby safeguarding against aneuploidy.
INCENP has strong connections to cancer and various chromosomal instability syndromes. Misregulation or mutation of INCENP can lead to an improper chromosomal segregation resulting in aneuploidy a hallmark of many cancer types. Its association with Aurora B and the other CPC components further implicates its role in tumorigenesis. Alterations in INCENP function could also contribute to disorders characterized by genomic instability highlighting its importance in cell division and genomic integrity.