Abcam, ab136648, Anti-ARL13B antibody [N295B/66]

Size: 100µg
Anti-ARL13B antibody [N295B/66] (ab136648) is a mouse monoclonal antibody detecting ARL13B in Western Blot, IHC-P . Suitable for Mouse, Rat . - Over 30 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:N295B/66,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Rat,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
What is this antibody validated in?
Anti-ARL13B antibody [N295B/66] (ab136648) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Mouse, Rat samples.
What is the molecular weight of ARL13B?
Anti-ARL13B [N295B/66] (ab136648) specifically detects a band for ARL13B (UniProt: Q3SXY8) at a molecular weight of 60kDa.
Trusted by the scientific community
Anti-ARL13B [N295B/66] (ab136648) was first used in a scientific publication in 2012 and has been cited over 30 times in peer-reviewed journals.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 6.97% L-Arginine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ARL13B also known as ADP-ribosylation factor-like protein 13B is a small GTPase with a molecular mass of approximately 45 kDa. This protein predominantly localizes to the ciliary membrane of cells a specific structure important for cell signaling in eukaryotic organisms. Beyond its association with the cilia ARL13B expression is notable in complex neuronal tissues and renal epithelial cells indicating its diverse roles in various physiological processes.
Biological function summary
ARL13B plays an essential role in the formation and maintenance of primary cilia which are key to cell signaling and sensory processing. ARL13B functions as part of a multiprotein complex within the ciliary membrane essential for the localization and distribution of other ciliary proteins. Through its GTPase activity ARL13B regulates ciliary dynamics and signaling pathways that are fundamental to proper cellular function and development.
Pathways
ARL13B modulates the Sonic Hedgehog (Shh) and Wnt signaling pathways both critical in developmental processes and tissue homeostasis. ARL13B interacts with several proteins in these pathways to control signal transduction such as Gli proteins in the Shh pathway and various cytoplasmic and nuclear factors in the Wnt pathway. ARL13B's role as a signaling regulator highlights its importance in maintaining cellular communication and function.
Mutations or dysregulation of ARL13B are associated with Joubert syndrome and Meckel-Gruber syndrome both developmental disorders affecting the brain and other organs. These conditions arise partly from disrupted cilia function linking ARL13B directly to cilia-related syndromes. In Joubert syndrome ARL13B interacts with proteins such as Cep290 and IFT88 emphasizing its involvement in the pathology of ciliary dysfunctions and providing a focal point for understanding and potentially targeting these disorders.